Pathological changes associated with white striping in broiler breast muscles
Pathological changes associated with white striping in broiler breast muscles
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DOI：
10.3382/ps.2012-02646
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发表时间：
2013-02-01
期刊：
影响因子：
4.4
通讯作者：
Owens, C. M.
中科院分区：
文献类型：
--
作者：
Kuttappan, V. A.;Shivaprasad, H. L.;Owens, C. M.
白色条纹是肉鸡中的一种病症，其特征在于在肉鸡胸片和大腿上出现与肌纤维方向平行的白色条纹。基于白色条纹强度的视觉评价，乳房鱼片可分为正常（NORM）、中度（MOD）和重度（SEV）类别。本研究旨在评价组织学变化的细节以及鱼片中发生的3度白色条纹的近似成分。在实验1中，从45日龄的肉鸡中收集每种程度的白色条纹（n = 20）的代表性胸片，大约2小时后死亡。从每个鱼片中获得2个骨骼肌样品，并固定在10%中性缓冲福尔马林中。为了识别和区分组织学变化，制备载玻片并使用苏木精和伊红、Masson三色和油红O染色剂染色。试验2从57日龄鸡中采集3度白色条纹样品进行工业分析。在MOD和SEV样本中观察到的主要组织病理学变化包括横纹丢失、纤维大小变异、絮状/空泡变性和纤维溶解、轻度矿化、偶尔再生（核排列和多核细胞）、单核细胞浸润、纤维变性和间质性炎症和纤维化。对显微镜下病变的变性和坏死、纤维化和变性进行目视评分。用于对样品进行评分的量表范围为0（正常）至3（严重）。随着白色条纹程度从NORM增加到SEV，退行性或坏死性病变、纤维化和纤维变性的平均评分增加（P < 0.05）。组织病理学研究结果与工业分析结果一致，证实肌肉脂肪和蛋白质含量随白色条纹程度的增加而分别增加（P < 0.05）和降低（P < 0.05）。总之，白色条纹中发生的组织病理学变化表明可能与鸟类生长速率增加相关的退行性肌病。
White striping is a condition in broiler chickens characterized grossly by the occurrence of white striations, seen parallel to the direction of muscle fibers, on broiler breast fillets and thighs. Based on visual evaluation of the intensity of white striping, breast fillets can be categorized into normal (NORM), moderate (MOD), and severe (SEV) categories. This study was undertaken to evaluate the details of changes in histology as well as proximate composition occurring in the fillets with respect to the 3 degrees of white striping. In experiment 1, representative breast fillets for each degree of white striping (n = 20) were collected from 45-d-old broilers, approximately 2 h postmortem. From each fillet, 2 skeletal muscle samples were obtained and fixed in 10% neutral buffered formalin. To identify and differentiate the histological changes, slides were prepared and stained using hematoxylin and eosin, Masson's Trichrome, and Oil Red O stains. In experiment 2, samples with 3 degrees of white striping were collected from 57-d-old birds for conducting proximate analysis. Major histopathological changes observed in the MOD and SEV samples consisted of loss of cross striations, variability in fiber size, floccular/vacuolar degeneration and lysis of fibers, mild mineralization, occasional regeneration (nuclear rowing and multinucleated cells), mononuclear cell infiltration, lipidosis, and interstitial inflammation and fibrosis. Microscopic lesions were visually scored for degeneration and necrosis, fibrosis, and lipidosis. The scale used to score the samples ranged from 0 (normal) to 3 (severe). There was an increase (P < 0.05) in mean scores for degenerative or necrotic lesions, fibrosis, and lipidosis as the degree of white striping increased from NORM to SEV. The results from the histopathological study were supported by the findings from proximate analysis confirming that the fat and protein contents of muscle increased (P < 0.05) and decreased (P < 0.05), respectively, as the degree of white striping increased. In conclusion, the histopathological changes occurring in white striping indicate a degenerative myopathy that could be associated with increased growth rate in birds.