PHARMACOLOGICAL CHARACTERIZATION OF AMINE RECEPTORS ON EMBRYONIC CHICK SENSORY NEURONS
PHARMACOLOGICAL CHARACTERIZATION OF AMINE RECEPTORS ON EMBRYONIC CHICK SENSORY NEURONS
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DOI：
10.1111/j.1476-5381.1984.tb10794.x
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发表时间：
1984-01-01
影响因子：
7.3
通讯作者：
DUNLAP, K
中科院分区：
文献类型：
--
作者：
CANFIELD, DR;DUNLAP, K
研究了去甲肾上腺素、多巴胺和5-羟色胺对离体鸡胚感觉神经元动作电位时程的影响。所有三种胺，如γ-氨基丁酸，减少了电流注入诱发的动作电位的持续时间。去甲肾上腺素诱导的动作电位时程下降的开始时间很快（小于1 s），恢复期取决于去甲肾上腺素的剂量。去甲肾上腺素的快速洗脱显示至少30 s的恢复时间，这与初始去甲肾上腺素浓度无关。多巴胺和5-羟色胺可模拟去甲肾上腺素对动作电位时程的影响。所有三种胺的ED 50约为1 μ M。在10 μ M的饱和浓度下，去甲肾上腺素比多巴胺和5-羟色胺更有效。饱和剂量的去甲肾上腺素和多巴胺或5-羟色胺是不加和的。对所有三种胺的反应受到拮抗剂的类似影响：它们被育亨宾、酚妥拉明、氟哌啶醇和米安色林拮抗，但不被普萘洛尔、哌唑嗪、多潘立酮、螺哌隆或麦角新碱拮抗。可乐定和甲苯噻嗪（α 2-肾上腺素受体激动剂）也没有效果。与胺相反，γ-氨基丁酸的饱和浓度与去甲肾上腺素的饱和浓度相加。对GABA的反应不被胺受体拮抗剂拮抗。这里描述的证据表明，胺和γ-氨基丁酸通过药理学上不同的膜受体减少感觉神经元动作电位持续时间。此外，很可能胺通过一类受体起作用，其药理学不同于经典的肾上腺素受体、多巴胺受体和5-羟色胺受体。
The effects of noradrenaline, dopamine and 5-hydroxytryptamine were investigated on the duration of the action potential of embryonic chick sensory neurones in vitro. All three amines, like gamma-aminobutyric acid, decreased the duration of the action potential evoked by current injection. The onset of the noradrenaline-induced decrease in action potential duration was fast (less than 1s) and the recovery phase was dependent upon the dose of noradrenaline applied. Rapid washout of the noradrenaline revealed a minimum 30s recovery time which was independent of the initial noradrenaline concentration. Dopamine and 5-hydroxytryptamine could mimic the effects of noradrenaline on action potential duration. The ED50 for all three amines was approximately 1 microM. At a saturating concentration of 10 microM, noradrenaline was more potent than dopamine and 5-hydroxytryptamine. Saturating doses of noradrenaline and dopamine or 5-hydroxytryptamine were not additive. Responses to all three amines were affected similarly by antagonists: they were antagonized by yohimbine, phentolamine, haloperidol and mianserin but not by propranolol, prazosin, domperidone, spiperone or methysergide. Clonidine and xylazine (alpha 2-adrenoceptor agonists) were also without effect. In contrast to the amines, saturating concentrations of gamma-aminobutyric acid were additive with those of noradrenaline. Responses to GABA were not antagonized by the amine receptor antagonists. The evidence described here suggests that the amines and gamma-aminobutyric acid acid decrease sensory neurone action potential duration via pharmacologically-distinct membrane receptors. In addition, it is likely that the amines are acting via a single class of receptor whose pharmacology is different from classical adrenoceptors, dopamine receptors and 5-hydroxytryptamine receptors.