Highly restricted expression cerebellar Purkinje cells
Highly restricted expression cerebellar Purkinje cells
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DOI：
10.1002/gene.20062
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发表时间：
2004-09-01
期刊：
影响因子：
1.5
通讯作者：
Huang, JD
中科院分区：
文献类型：
--
作者：
Zhang, XM;Ng, AHL;Huang, JD
浦肯野神经元是小脑皮层最迷人的组成部分之一，参与运动学习、运动协调和认知功能。浦肯野细胞蛋白2（Pcp 2/L7）表达高度限于浦肯野细胞和视网膜双极细胞，在那里它已被利用来实现高度特异性的Cre重组酶介导的位点特异性重组。先前的研究表明，小鼠携带Cre的cDNA插入到一个小的2.88 kb的Pcp 2 DNA片段产生的Cre转基因表达Cre在浦肯野细胞，然而，一些Cre活性也观察到以外的目标组织。在这里，我们使用Red介导的重组工程将Cre cDNA插入到携带完整Pcp 2基因的173-kb BAC中，并对所得的BAC/Cre转基因小鼠的Cre表达进行表征。我们发现BAC/Cre转基因小鼠在浦肯野细胞和双极细胞中有专属的Cre表达。这些小鼠将促进浦肯野细胞和视网膜双极细胞特异性遗传操作。(C)2004 Wiley-Liss，Inc.
The Purkinje neuron, one of the most fascinating components of the cerebellar cortex, is involved in motor learning, motor coordination, and cognitive function. Purkinje cell protein 2 (Pcp2/L7) expression is highly restricted to Purkinje and retinal bipolar cells, where it has been exploited to enable highly specific, Cre recombinase-mediated, site-specific recombination. Previous studies showed that mice carrying a Cre transgene produced by insertion of Cre cDNA into a small 2.88-kb Pcp2 DNA fragment expressed Cre in Purkinje cells; however, some Cre activity was also observed outside the target tissues. Here, we used Red-mediated recombineering to insert Cre cDNA into a 173-kb BAC carrying the entire intact Pcp2 gene, and characterize the resultant BAC/Cre transgenic mice for Cre expression. We show that BAC/Cre transgenic mice have exclusive Cre expression in Purkinje and bipolar cells and nowhere else. These mice will facilitate Purkinje cell and retinal bipolar cell-specific genetic manipulation. (C) 2004 Wiley-Liss, Inc.