DO beta: a new beta chain gene in HLA‐D with a distinct regulation of expression.
DO beta: a new beta chain gene in HLA‐D with a distinct regulation of expression.
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DO beta：HLA-D 中的一种新的 β 链基因，具有独特的表达调节。
DOI：
10.1002/j.1460-2075.1985.tb04012.x
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Eric O Long
中科院分区：
文献类型：
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作者：
C. Tonnelle;R. Demars;Eric O Long
人类主要组织相容性复合体的HLA-D区域编码DP、DQ和DR II类抗原的α和β链的基因。从由具有单一HLA单倍型的突变B-细胞系的mRNA构建的文库中分离编码新的II类β链（命名为DO）的cDNA克隆。对编码DR 1、DQw 1、DPw 2和推定DO抗原的四种同型β链的完整cDNA克隆进行测序。通过与HLA缺失突变体的DNA杂交，将DO β基因定位在D区。DO β mRNA在B-细胞系中表达较低，但在其他II类基因表达缺失的突变体系中仍存在。与其他II类基因不同，DO β在成纤维细胞系中不受γ-干扰素诱导。DO β基因与DP β、DQ β和DR β基因的核苷酸趋异模式不同。DO β的独立进化和表达表明它可能是功能不同的II类分子的一部分。
The HLA‐D region of the human major histocompatibility complex encodes the genes for the alpha and beta chains of the DP, DQ and DR class II antigens. A cDNA clone encoding a new class II beta chain (designated DO) was isolated from a library constructed from mRNA of a mutant B‐cell line having a single HLA haplotype. Complete cDNA clones encoding the four isotypic beta chains of the DR1, DQw1, DPw2 and putative DO antigens were sequenced. The DO beta gene was mapped in the D region by hybridization with DNA of HLA‐deletion mutants. DO beta mRNA expression is low in B‐cell lines but remains in mutant lines which have lost expression of other class II genes. Unlike other class II genes DO beta is not induced by gamma‐interferon in fibroblast lines. The DO beta gene is distinct from the DP beta, DQ beta and DR beta genes in its pattern of nucleotide divergence. The independent evolution and expression of DO beta suggest that it may be part of a functionally distinct class II molecule.