Temperature and pH Affect the Production of Bacterial Biofilm
Temperature and pH Affect the Production of Bacterial Biofilm
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DOI：
10.1007/s12223-010-0012-y
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发表时间：
2010-01-01
影响因子：
2.6
通讯作者：
Stefkovicova, M.
中科院分区：
文献类型：
--
作者：
Hostacka, A.;Ciznar, I.;Stefkovicova, M.
不同的培养温度（30和37摄氏度）和介质的pH值（5.5，7.5，8.5）对生物膜生产的影响进行了比较，在铜绿假单胞菌，肺炎克雷伯氏菌，霍乱弧菌非O 1和O 1使用结晶紫测试的生物膜的定量生产的估计。与30 ℃下的生物膜产量相比，在37 ℃下在8种测试菌株（铜绿假单胞菌3株、肺炎克雷伯菌2株、霍乱弧菌非O 1 2株和霍乱弧菌O 1 1 1株）中观察到生物膜产量减少（46.4- 98.4%）。另一方面，5株菌株（铜绿假单胞菌1，克雷伯氏菌1，肺炎球菌3，非O 1霍乱弧菌1）在这些条件下表现出更高的生物膜产生（103- 143%）。然而，该差异不显著（p = 0.196）。使用所有测试的培养基，增加的pH导致更高的生物膜产生。在铜绿假单胞菌中，与pH 5.5相比，pH 8.5下的生物膜产量为139- 244%，pH 7.5下为136- 164%。同样，在K.对于肺炎球菌，在pH 8.5下，生物膜产量增加至151- 319%，而随着pH降至7.5，与pH 5.5相比，生物膜产量为113- 177%。在非O 1和O 1霍乱弧菌中，生物膜产量在pH 8.5时达到204- 329%，在pH 7.5时达到123- 316%（与pH 5.5时的产量相比）。在pH从5.5变化到7.5时，生物膜产生的增加平均为169%（p = 0.001），pH从5.5升高到8.5引起229%的平均差异（p = 0.001）。
The effect of different cultivation temperatures (30 and 37 degrees C) and pH of the media (5.5, 7.5, 8.5) on the biofilm production was compared in Pseudomonas aeruginosa, Klebsiella pneumoniae, and Vibrio cholerae non-O1 and O1 using the crystal-violet test for estimation of quantitative production of the biofilm. Decrease (46.4-98.4 %) in the biofilm production was observed at 37 degrees C in 8 of the tested strains (P. aeruginosa three strains, K pneumoniae two, V. cholerae non-O1 two, and V. cholerae O1 one strain) compared with the production at 30 degrees C. On the other hand, five strains (P. aeruginosa 1, K. pneumoniae 3, V. cholerae non-O1 1) exhibited under these conditions a higher biofilm production (103-143 %). However, this difference was not significant (p = 0.196). Increased pH lead to a higher biofilm production using all media tested. In P. aeruginosa the biofilm production at pH 8.5 was 139-244 %, at pH 7.5 136-164 % in comparison with pH 5.5. Similarly, in K. pneumoniae the biofilm production increased to 151-319 % at pH 8.5 while with the drop of pH to 7.5 the biofilm production was 113-177 % compared with pH 5.5. In V. cholerae non-O1 and O1 the biofilm production reached 204-329 % at pH 8.5, and 123-316 % at pH 7.5 (compared with the production at pH 5.5). An increase in biofilm production represented an average of 169 % (p = 0.001) at pH change from 5.5 to 7.5, with the rise of pH from 5.5 to 8.5 caused an average difference of 229 % (p = 0.001).