Rescue of Rinderpest virus from cloned cDNA
Rescue of Rinderpest virus from cloned cDNA
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DOI：
10.1128/jvi.71.2.1265-1271.1997
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发表时间：
1997-02-01
影响因子：
5.4
通讯作者：
Barrett, T
中科院分区：
文献类型：
--
作者：
Baron, MD;Barrett, T
牛瘟病毒是一种麻疹病毒，是一种广泛而重要的牛疾病的病原体。病毒基因组是负链RNA。我们已经构建了含有由报告基因分开的病毒5'和3'末端的cDNA拷贝的质粒，并且已经表明这些模型基因组的抗原组正义RNA转录物可以被辅助病毒（牛瘟病毒和相关的麻疹病毒）复制、转录和包装。此外，这些基因组类似物可以通过使用表达T7 RNA聚合酶的重组牛痘病毒（MVA-T7）由cDNA克隆表达的病毒蛋白复制和转录。使用后一种系统，我们从RBOK疫苗株基因组的全长cDNA拷贝中拯救了活牛瘟病毒。重组病毒在组织培养中的生长似乎与原始病毒相同。
Rinderpest virus is a morbillivirus and is the causative agent of a widespread and important disease of cattle. The viral genome is a single strand of RNA in the negative sense. We have constructed plasmids containing cDNA copies of the 5' and 3' termini of the virus separated by a reporter gene and have shown that antigenome-sense RNA transcripts of these model genomes can be replicated, transcribed, and packaged by helper virus, both rinderpest virus and the related measles virus. Further, these genome analogs can be replicated and transcribed by viral proteins expressed from cDNA clones by using a recombinant vaccinia virus expressing T7 RNA polymerase (MVA-T7). Using this latter system, we have rescued live rinderpest virus from a full-length cDNA copy of the genome of the RBOK vaccine strain. The recombinant virus appears to grow in tissue culture identically to the original virus.