SPLEEN-DERIVED GROWTH-FACTOR, SDGF-3, IS IDENTIFIED AS KERATINOCYTE GROWTH-FACTOR (KGF)
SPLEEN-DERIVED GROWTH-FACTOR, SDGF-3, IS IDENTIFIED AS KERATINOCYTE GROWTH-FACTOR (KGF)
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DOI：
10.1016/0014-5793(93)80956-u
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发表时间：
1993-08-09
期刊：
影响因子：
3.5
通讯作者：
MITSUI, Y
中科院分区：
文献类型：
--
作者：
SUZUKI, M;ITOH, T;MITSUI, Y
通过肝素亲和层析、阳离子交换层析和凝胶过滤层析的组合，从牛脾中部分纯化出大鼠肝细胞的肝素结合丝裂原大鼠。除了刺激大鼠肝细胞外，这种被命名为脾源性生长因子-3 (SDGF-3) 的因子对小鼠表皮角质形成细胞显示出促有丝分裂活性，但对小鼠成纤维细胞则没有。其明显的上皮特异性和肝素结合特性与角质形成细胞生长因子（KGF）相对应。这些发现，加上 SDGF-3 的促有丝分裂活性被 KGF 特异性中和单克隆抗体消除的事实，将这种源自牛脾的肝细胞有丝分裂原鉴定为 KGF。
A heparin-binding mitogen rat for rat hepatocytes was partially purified from bovine spleen by a combination of heparin-affinity, cation-exchange and gel-filtration chromatography. Besides stimulating rat hepatocytes, this factor, which was designated spleen-derived growth factor-3 (SDGF-3), exhibited mitogenic activity for mouse epidermal keratinocytes but not mouse fibroblasts. Its apparent epithelial specificity and heparin-binding properties corresponded to those of keratinocyte growth factor (KGF). These findings, together with the fact that the mitogenic activity of SDGF-3 was abolished by a neutralizing monoclonal antibody specific for KGF, identify this bovine spleen-derived hepatocyte mitogen as KGF.