Efficacy of common disinfectant/cleaning agents in inactivating murine norovirus and feline calicivirus as surrogate viruses for human norovirus
Efficacy of common disinfectant/cleaning agents in inactivating murine norovirus and feline calicivirus as surrogate viruses for human norovirus
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DOI：
10.1016/j.ajic.2015.06.021
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发表时间：
2015-11-01
影响因子：
4.9
通讯作者：
Isaac-Renton, Judith
中科院分区：
文献类型：
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作者：
Chiu, Stephanie;Skura, Brenton;Isaac-Renton, Judith
背景：使用替代病毒鼠诺如病毒 (MNV-1) 和猫杯状病毒 (FCV) 测定医疗机构中常用的次氯酸钠、加速过氧化氢 (AHP) 和季铵化合物 (QUAT) 的消毒效果。方法：将已知浓度的病毒悬浮液（有或没有土壤负荷）在湿负荷或干负荷条件下沉积到不锈钢盘上，并暴露于规定浓度的消毒剂/清洁剂中，以进行消毒。使用定量载体测试 (QCT-2) 方法的 1 分钟、5 分钟或 10 分钟接触时间。通过噬菌斑测定确定病毒灭活情况。结果：在 1 分钟的暴露时间下，2,700 ppm 的次氯酸钠能够灭活 MNV-1 和 FCV，减少量 >5 log10。 10 分钟后，MNV-1 在 35,000 ppm 的浓度下被 AHP 灭活，而 FCV 在 3,500 ppm 的浓度下灭活。 2,800 ppm 的 QUAT 不会使 MNV-1 失活。含有 2,000 ppm QUAT 和 70% 乙醇的 QUAT 醇制剂在 5 分钟后可有效灭活 MNV-1，但仅产生
Background: The efficacies of disinfection by sodium hypochlorite, accelerated hydrogen peroxide (AHP), and quaternary ammonium compound (QUAT) commonly used in health care facilities were determined using the surrogate viruses murine norovirus (MNV-1) and feline calicivirus (FCV).Methods: A virus suspension of known concentration (with or without a soil load) was deposited onto stainless steel discs under wet or dry load conditions and exposed to defined concentrations of the disinfectant/cleaning agent for 1-, 5-, or 10-minute contact time using the quantitative carrier test (QCT-2) method. Virus inactivation was determined by plaque assay.Results: At an exposure time of 1 minute, sodium hypochlorite at 2,700 ppm was able to inactivate MNV-1 and FCV with a >5 log10 reduction. After 10 minutes, MNV-1 was inactivated by AHP at 35,000 ppm, whereas FCV was inactivated at 3,500 ppm. MNV-1 was not inactivated by QUAT at 2,800 ppm. A QUAT-alcohol formulation containing 2,000 ppm QUAT and 70% ethanol was effective in inactivating MNV-1 after 5 minutes, but resulted in only a