APIGENIN INDUCES MORPHOLOGICAL-DIFFERENTIATION AND G2-M ARREST IN RAT NEURONAL CELLS
APIGENIN INDUCES MORPHOLOGICAL-DIFFERENTIATION AND G2-M ARREST IN RAT NEURONAL CELLS
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DOI：
10.1006/bbrc.1994.2498
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发表时间：
1994-10-28
影响因子：
3.1
通讯作者：
SAKAI, T
中科院分区：
文献类型：
--
作者：
SATO, F;MATSUKAWA, Y;SAKAI, T
香精是可食用植物的色素。我们最近报道，大多数黄酮类化合物诱导人癌细胞GI停滞，而染料木黄酮（染料木黄酮）特异性地抑制其细胞周期在G2-M期。本研究发现，芹菜素（一种黄酮类化合物）可抑制B104大鼠神经细胞的增殖，流式细胞仪分析表明，芹菜素可使细胞周期阻滞于G2-M期。当从培养基中去除芹菜素时，这种作用是剂量依赖性和可逆的。显微镜观察发现，芹菜素诱导B104细胞形态分化，即神经突起延长和树枝状化。这是首次报道芹菜素通过G2-M期阻滞抑制恶性肿瘤细胞增殖并诱导形态分化。(C)1994年出版社出版。
Flavonoids are pigments of edible plants. We have recently reported that most flavonoids induce GI arrest in human cancer cells, and that genistein (an isoflavone) specifically inhibits their cell cycle at G2-M phase. In the present study, apigenin (a flavone) was found to inhibit the proliferation of B104 rat neuronal cells, and flow-cytometric analysis showed that apigenin arrested their cell cycle at G2-M phase. This effect was dose-dependent and reversible when apigenin was removed from the culture medium. Microscopic observation showed that apigenin induced morphological differentiation, that is, elongation and arborization of neurites in B104 cells. This is the first report to show that apigenin inhibited the proliferation of malignant tumor cells by G2-M arrest and induced morphological differentiation. (C) 1994 Academic Press, Inc.