Multiplexed sensing of mercury(II) and silver(I) ions: A new class of DNA electrochemiluminescent-molecular logic gates
Multiplexed sensing of mercury(II) and silver(I) ions: A new class of DNA electrochemiluminescent-molecular logic gates
复制标题
汞 (II) 和银 (I) 离子的多重传感：一类新型 DNA 电化学发光分子逻辑门
DOI：
10.1016/j.bios.2011.02.003
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发表时间：
2011-04-15
影响因子：
12.6
通讯作者：
Ding, Tianrong
中科院分区：
文献类型：
--
作者：
Li, Xuemei;Sun, Li;Ding, Tianrong
Most of the DNA logic gates employ fluorescent or colorometric signals as their outputs, which were limited by the cumbersome handling procedures, lack of portability and lower sensitivity. To the best of our knowledge, the logic gates with electrochemiluminescent (ECL) signal as their outputs have not been reported. In response, we report here the construction of DNA molecular logic gates that produce ECL signals as their outputs, having the advantages of versatility, low background and simplified optical setup. The logic gates are based on the T-rich or C-rich oligonucleotides for the selective analysis of Hg2+ and Ag+ ions using energy or electron transfer-quenching path. Efficient and stable quenching of ECL of Ru bis(2,2'-bipyridine) (2,2'-bipyridine-4,4'-dicarboxylic acid) N-hydroxysuccinimide ester by oxidizing ferrocene at the Au electrode enabled us to use Hg2+ and Ag+ ions as inputs that activate logic gates, and to execute ECL of Ru(II) as readout signals for logic gate operations. (C) 2011 Elsevier B.V. All rights reserved.
