NEUTRALIZATION OF BOVINE HERPESVIRUS TYPE-4 BY PAIRS OF MONOCLONAL-ANTIBODIES RAISED AGAINST 2 GLYCOPROTEINS AND IDENTIFICATION OF ANTIGENIC DETERMINANTS INVOLVED IN NEUTRALIZATION
NEUTRALIZATION OF BOVINE HERPESVIRUS TYPE-4 BY PAIRS OF MONOCLONAL-ANTIBODIES RAISED AGAINST 2 GLYCOPROTEINS AND IDENTIFICATION OF ANTIGENIC DETERMINANTS INVOLVED IN NEUTRALIZATION
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DOI：
10.1099/0022-1317-71-3-647
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发表时间：
1990-03-01
影响因子：
3.8
通讯作者：
PASTORET, PP
中科院分区：
文献类型：
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作者：
DUBUISSON, J;GUILLAUME, J;PASTORET, PP
在受感染的牛中，4 型牛疱疹病毒 (BHV-4) 会诱导中和抗体水平较低或不存在此类抗体的免疫反应。为了研究这一现象，在中和测试中使用了针对先前鉴定的两种 BHV-4 糖蛋白（150K/120K/51K 和 120K/16.cntdot.5K）的单克隆抗体 (MAb)。除 MAb 16 外，没有任何 MAb 可以单独中和；针对 150K/120K/51K 和 120K/16.cntdot.5K 糖蛋白的单克隆抗体对能够中和 BHV-4 感染性。参与中和的单克隆抗体用于竞争性结合测定，以鉴定与 BHV-4 中和相关的表位。这些 MAb 显示出低亲和力和弱中和活性，并且它们部分减少了 BHV-4 对细胞的附着。这些结果表明，参与病毒感染性的 BHV-4 糖蛋白结构域很少暴露于免疫系统。
In infected cattle, bovine herpesvirus type 4 (BHV-4) induces an immune response with low neutralizing antibody levels or in the absence of such antibodies. For the study of the phenomenon, monoclonal antibodies (MAbs) raised against two BHV-4 glyco-proteins identified previously (150K/120K/51K and 120K/16.cntdot.5K) were used in neutralization tests. None of the MAbs except for MAb 16 could neutralize alone; pairs of MAbs against the 150K/120K/51K and 120K/16.cntdot.5K glycoproteins were able to neutralize BHV-4 infectivity. MAbs involved in neutralization were used in competitive binding assays to identify epitopes relevant for BHV-4 neutralization. These MAbs showed a low avidity and a weak neutralizing activity, and they partially decreased BHV-4 attachment to cells. These results suggest that the BHV-4 glycoprotein domains involved in viral infectivity are poorly exposed to the immune system.