NEGATIVE SURFACE CHARGE OF ABNORMAL HUMAN TROPHOBLAST
NEGATIVE SURFACE CHARGE OF ABNORMAL HUMAN TROPHOBLAST
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异常人类滋养细胞的负表面电荷
DOI：
10.1267/ahc.14.554
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
S. Sakamoto
中科院分区：
文献类型：
--
作者：
K. Kawagoe;T. Kawana;S. Sakamoto
为了解异常滋养细胞表面负电荷的特征，揭示其生物学作用，以阳离子化铁蛋白为超微结构标记，对自然流产、部分性葡萄胎和完全性葡萄胎的滋养细胞表面负电荷进行了电镜观察。的程度和分布的负电荷进行了研究，在比较与表面超微结构和表面糖蛋白鉴定钌红staining.In自然流产的情况下，不同的网站的人滋养层的表面结构，最好的相关性与合胞体滋养层本身的变性程度。对于自然流产的绒毛，在常规电子显微镜下是正常的，观察到表面糖蛋白在滋养层表面上形成明显的聚集体，伴随着表面负电荷的减少。在严重退化的体系中，表面糖蛋白的基本结构随着表面负电荷的显著减少而丧失。这一发现似乎显示了滋养层表面负电荷的可能作用，即在静电排斥力的作用下，膜结合糖蛋白可以保持其正常的规则位置，因此，滋养层的膜特异性可以表达。当完全性葡萄胎和部分性葡萄胎都用钌红染色和阳离子化铁蛋白电镜观察时，完全性葡萄胎与部分性葡萄胎的本质区别在于前者类似于正常绒毛，而后者类似于自然流产。
In order to characterize the negative surface charge of the abnormal human trophoblast and to cast light on its biological role, the negative charges on the trophoblastic surface of spontaneous abortion, partial mole and complete mole were investigated by electron microscopy using cationized ferritin as an ultrastructural marker. The degree and distribution of negative charge were also studied in comparison with surface ultrastructure and surface glycoprotein identified by ruthenium red staining.In a case of spontaneous abortion, the surface structure of the human trophoblast differed from site to site, correlating best with the degree of degeneration of the syncytiotrophoblast itself. For villi from a spontaneous abortion, which were otherwise normal in routine electron microscopy, the surface glycoprotein was observed to make prominent aggregates on the trophoblastic surface in concomitance with the reduced negative surface charge. In a severely degenerated system, the basic structure of the surface glycoprotein was lost in accordance with significant decrease of the negative surface charge. This finding seems to show the possible role of the negative surface charge of the trophoblast, that with the electrostatic repulsive forces, the membrane-bound glycoproteins can maintain their normal regular positions and, as a result, membrane specificity of the trophoblast can be expressed. When both complete hydatidiform mole and partial mole were observed by electron microscopy using ruthenium red staining and cationized ferritin, the complete mole differed essentially from the partial mole in that the former resembled normal villi while the latter resembled spontaneous abortion.