Bioaugmentation with a pyridine-degrading bacterium in a membrane bioreactor treating pharmaceutical wastewater.
Bioaugmentation with a pyridine-degrading bacterium in a membrane bioreactor treating pharmaceutical wastewater.
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DOI：
10.1016/s1001-0742(12)60278-2
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发表时间：
2013-11
影响因子：
6.9
通讯作者：
Donghui Wen;Jing Zhang;R. Xiong;Rui Liu;Lu-jun Chen
中科院分区：
文献类型：
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作者：
Donghui Wen;Jing Zhang;R. Xiong;Rui Liu;Lu-jun Chen
将吡啶作为其唯一碳源和氮源的细菌菌株反硝化副球菌W12添加到膜生物反应器（MBR）中以增强对制药废水的处理。研究的处理效率表明，生物强化和非生物强化MBR对化学需氧量、总氮和总磷的去除率相似，然而，在生物强化反应器中吡啶的去除率显着提高。当水力停留时间为60 h、吡啶进水浓度为250~500 mg/L时，不添加W12时吡啶的平均出水浓度为57.2 mg/L，而添加W12后吡啶的平均降解浓度为10.2 mg/L。采用聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）分析生物强化处理过程中活性污泥的细菌群落结构。结果表明，W12接种物扭转了微生物群落多样性的下降，但在废水处理过程中，原污泥与生物强化后污泥细菌群落结构的相似性稳步下降。从 DGGE 凝胶回收的 DNA 测序表明，黄杆菌科 (Flavobacteriaceesp.)、鞘氨醇菌属 (Sphingobium sp.)、丛毛单胞菌属 (Comamonassp.) 和生丝微菌属 (Hyphomicrobium sp.)。是生物增强 MBR 中细菌群落中按时间顺序排列的优势生物体。这意味着生物增强作用受到恶劣环境中整个细菌群落结构调整的影响，而不仅仅是由于添加的细菌的降解性能。
The bacterial strainParacoccus denitrificansW12, which could utilize pyridine as its sole source of carbon and nitrogen, was added into a membrane bioreactor (MBR) to enhance the treatment of a pharmaceutical wastewater. The treatment efficiencies investigated showed that the removal of chemical oxygen demand, total nitrogen, and total phosphorus were similar between bioaugmented and non-bioaugmented MBRs, however, significant removal of pyridine was obtained in the bioaugmented reactor. When the hydraulic retention time was 60 hr and the influent concentration of pyridine was 250–500 mg/L, the mean effluent concentration of pyridine without adding W12 was 57.2 mg/L, while the pyridine was degraded to an average of 10.2 mg/L with addition of W12. The bacterial community structure of activated sludge during the bioaugmented treatment was analyzed using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). The results showed that the W12 inoculum reversed the decline of microbial community diversity, however, the similarity between bacterial community structure of the original sludge and that of the sludge after bioaugmentation decreased steadily during the wastewater treatment. Sequencing of the DNA recovered from DGGE gel indicated thatFlavobacteriaceaesp.,Sphingobiumsp.,Comamonassp., andHyphomicrobiumsp. were the dominant organisms in time sequence in the bacterial community in the bioaugmented MBR. This implied that the bioaugmentation was affected by the adjustment of whole bacterial community structure in the inhospitable environment, rather than being due solely to the degradation performance of the bacterium added.