Do assorted approaches aid in estimation of uronic acids? Case studies on Tinospora sinensis polysaccharides
Do assorted approaches aid in estimation of uronic acids? Case studies on Tinospora sinensis polysaccharides
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DOI：
10.1016/j.ijbiomac.2014.07.010
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发表时间：
2014-09-01
影响因子：
8.2
通讯作者：
Tripathi, Y. C.
中科院分区：
文献类型：
--
作者：
Kumar, Vineet;Nagar, Shipra;Tripathi, Y. C.
含有糖醛酸的多糖具有重要的生物学意义。已经探索了使用不同色原的比色法用于多糖中的糖醛酸估计，但是当中性糖大量存在时，这些方法克服了许多困难。对从具有显着治疗意义的青牛胆中分离得到的冷水可溶性多糖（CWSP）和热水可溶性多糖（HWSP）的糖醛酸含量进行了研究。利用咔唑、3-苯基苯酚和3，5-二甲基苯酚（NH3）的分光光度法对糖醛酸进行了定量测定。半乳糖醛酸在CWSP和HWSP中的含量分别为1.41%和1.20%。这些发现揭示了色原的功效，也揭示了它们在多大程度上可以依赖。实验结果还表明，该试剂对咔唑和间苯基苯酚有较高的专一性和选择性。然而，尽管耗时，GLC分析仍然是精确估计糖醛酸的最佳方法。此外，糖醇乙酸酯的GLC分析表明CWSP中摩尔比为0.178、0.259、0.140、1.045和98.377的L-鼠李糖、L-阿拉伯糖、D-甘露糖、D-半乳糖和D-葡萄糖的单糖组成，以及CWSP中摩尔比为0.608、0.628、0.978、0.979和0.978的L-鼠李糖、L-阿拉伯糖、D-木糖、D-甘露糖、D-半乳糖和D-葡萄糖的单糖组成。HWSP分别为0.227、0.111和97.448。(C)2014爱思唯尔有限公司版权所有。
Polysaccharides containing uronic acids are of immense biological significance. Colorimetric methods using different chromogens have been explored for uronic acid estimation in polysaccharides but these methods counter numerous difficulties, when neutral sugars are present in substantial amount. The uronic acid content of cold water-soluble polysaccharide (CWSP) and hot water-soluble polysaccharide (HWSP) isolated from Tinospora sinensis, widely known for marked therapeutic significance has been studied. The uronic acid was quantitatively estimated by spectrophotometric methods using carbazole, 3-phenylphenol and 3,5-dimethylphenol (DMP) vis-a-vis gas-liquid chromatography (GLC) analysis. Galacturonic acid in CWSP and HWSP was estimated as 1.41% and 1.20% respectively. The findings bring to light, the efficacy of chromogens and also reveal to what extent, they could be relied upon. The results also indicated higher specificity and selectivity of DMP over carbazole and 3-phenylphenol. However, GLC analysis despite time consuming remains the finest method for precise estimation of uronic acids. Further, GLC analysis of alditol acetates indicated the monosugar composition of L-rhamnose, L-arabinose, D-mannose, D-galactose and D-glucose in molar ratio 0.178, 0.259, 0.140, 1.045 and 98.377 in CWSP and L-rhamnose, L-arabinose, D-xylose, D-mannose, D-galactose and D-glucose in molar ratio 0.608, 0.628, 0.978, 0.227, 0.111 and 97.448 in HWSP respectively. (C) 2014 Elsevier B.V. All rights reserved.