Simple gas chromatography analysis of faecal butyrate: Application to patients at risk of pouchitis
Simple gas chromatography analysis of faecal butyrate: Application to patients at risk of pouchitis
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DOI：
10.1515/cclm.2001.005
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发表时间：
2001-01-01
影响因子：
6.8
通讯作者：
Degand, PM
中科院分区：
文献类型：
--
作者：
Renom, G;Bulois, P;Degand, PM
尽管袋炎是采用回肠-肛门吻合术治疗溃疡性结肠炎患者最常见和最麻烦的并发症，但目前还没有生物标志物来监测该疾病的预后。由于已经注意到患有袋炎患者的粪便丁酸盐减少，我们开发了一种简单的气相色谱法来定量粪便水中的丁酸盐。本试验以乙醚萃取为基础，以甲基丙烯酸为内标。我们证明，当这项技术应用于11例回肠-袋肛门吻合术后的第一年，丁酸盐是有效的测量。我们还观察到，丁酸盐的浓度显著降低的病人谁继续发展一个袋炎。
In spite of the fact that pouchitis is the most frequently occurring and troublesome complication found in patients treated by ileo-anal anastomosis for ulcerative colitis, no biological marker currently exists to monitor the outcome of the disease. Since it has been noted faecal butyrate is reduced in patients with pouchitis, we developed a simple gas chromatography method to quantify butyrate in faecal water. This test is based on diethyl ether extraction with the use of methacrylic acid as an internal standard. We demonstrated that butyrate was effectively measured when this technique was applied to eleven patients with ileal-pouch anal anastomosis within the first year after the closure of their ileostomy. We also observed a noticeable reduction in the concentration of butyrate in patients who went on to develop a pouchitis.