Scaffolding of a bacterial genome using MinION nanopore sequencing.
Scaffolding of a bacterial genome using MinION nanopore sequencing.
复制标题
DOI：
10.1038/srep11996
复制
发表时间：
2015-07-07
影响因子：
4.6
通讯作者：
Stenberg P
中科院分区：
文献类型：
--
作者：
Karlsson E;Lärkeryd A;Sjödin A;Forsman M;Stenberg P
第二代测序使基因组研究发生了革命性的变化。然而，大多数基因组包含的重复DNA元件比典型测序仪所能获得的读取长度更长，因此不容易解析几个产生的重叠群的基因组顺序。提供更长读取时间的新一代测序仪正在出现，特别是太平洋生物科学(PacBio)RS II系统和Minion系统，该系统由牛津纳米孔技术公司于2014年初通过早期访问计划发布。后者具有非常有利的便携性，通过测量单链DNA分子通过纳米孔转移时离子电流的变化来对样品进行排序。我们表明，Minion系统产生了具有高映射性的长读取，可用于构建细菌基因组，尽管目前产生的错误率比PacBio读出要高得多。随着进一步的发展，我们预计Minion将不仅用于组装基因组，还将用于快速检测生物体，可能是在现场。
Second generation sequencing has revolutionized genomic studies. However, most genomes contain repeated DNA elements that are longer than the read lengths achievable with typical sequencers, so the genomic order of several generated contigs cannot be easily resolved. A new generation of sequencers offering substantially longer reads is emerging, notably the Pacific Biosciences (PacBio) RS II system and the MinION system, released in early 2014 by Oxford Nanopore Technologies through an early access program. The latter has highly advantageous portability and sequences samples by measuring changes in ionic current when single-stranded DNA molecules are translocated through nanopores. We show that the MinION system produces long reads with high mapability that can be used for scaffolding bacterial genomes, despite currently producing substantially higher error rates than PacBio reads. With further development we anticipate that MinION will be useful not only for assembling genomes, but also for rapid detection of organisms, potentially in the field.
登录
查看更多内容
影响因子：
46.9
作者：
Loman, Nicholas J.;Misra, Raju V.;Pallen, Mark J.
通讯作者：
Pallen, Mark J.
DOI：
10.1128/genomea.00986-14
发表时间：
2014-09-01
期刊：
GENOME ANNOUNCEMENTS
影响因子：
--
作者：
Terabayashi, Yasunobu;Juan, Ayaka;Hirano, Takashi
通讯作者：
Hirano, Takashi
影响因子：
6.7
作者：
Larsson P;Elfsmark D;Svensson K;Wikström P;Forsman M;Brettin T;Keim P;Johansson A
通讯作者：
Johansson A
影响因子：
56.9
作者：
Eid, John;Fehr, Adrian;Turner, Stephen
通讯作者：
Turner, Stephen
影响因子：
9.2
作者：
Quick J;Quinlan AR;Loman NJ
通讯作者：
Loman NJ