Factors That Influence the Quality of RNA From the Pancreas of Organ Donors
Factors That Influence the Quality of RNA From the Pancreas of Organ Donors
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DOI：
10.1097/mpa.0000000000000717
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发表时间：
2017-02-01
期刊：
影响因子：
2.9
通讯作者：
Kaddis, John S.
中科院分区：
文献类型：
--
作者：
Philips, Tiffany;Kusmartseva, Irina;Kaddis, John S.
目的：由于生理和后勤方面的考虑，从用于研究的已故捐赠者的组织或器官中获得高质量的RNA可能具有挑战性。方法：对236例器官供者的胰腺样本进行了RNA完整性数（RIN）测定，并用于区分高（>= 6.5）和低（>= 6.5）。
Objectives: Attaining high-quality RNA fromthe tissues or organs of deceased donors used for research can be challenging due to physiological and logistical considerations. In this investigation,Methods: RNA Integrity Number (RIN) was determined in pancreatic samples from 236 organ donors and used to define high (>= 6.5) and low (