Antibiotic resistance genes in treated wastewater and in the receiving water bodies: A pan-European survey of urban settings
Antibiotic resistance genes in treated wastewater and in the receiving water bodies: A pan-European survey of urban settings
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DOI：
10.1016/j.watres.2019.06.039
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发表时间：
2019-10-01
期刊：
影响因子：
12.8
通讯作者：
Berendonk, Thomas U.
中科院分区：
文献类型：
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作者：
Cacace, Damiano;Fatta-Kassinos, Despo;Berendonk, Thomas U.
抗生素耐药基因(ARGs)在污水处理过程中的去向、在处理过程中的持久性以及对接收水体的潜在影响日益受到公众的关注。在这项研究中，我们使用定量聚合酶链式反应(QPCR)检测了来自10个不同欧洲国家的16个污水处理厂(WWTP)出水中9个ARGs和1个1类整合子相关整合酶基因的丰度。为了评估对接收水体的影响，还分析了后者的基因丰度，在所分析的9个ARG中，有6个在所有污水和河流水质样本中检测到。在所量化的基因中，intI1和sul1含量最高。我们的结果表明，欧洲污水处理厂有助于浓缩接收水体中的阻力层，其具体影响取决于出水负荷和当地的水文条件。研究发现，污水处理厂出水中的ARGs浓度与所实施的生物处理步骤数呈负相关，这为污水处理厂的管理提供了一个可能的选择。此外，本研究还确定bla(OXA-58)可能是一个抗性基因，可用于未来研究污水处理厂对其接收水的影响。(C)2019年提交人。爱思唯尔有限公司出版。
There is increasing public concern regarding the fate of antibiotic resistance genes (ARGs) during wastewater treatment, their persistence during the treatment process and their potential impacts on the receiving water bodies. In this study, we used quantitative PCR (qPCR) to determine the abundance of nine ARGs and a class 1 integron associated integrase gene in 16 wastewater treatment plant (WWTP) effluents from ten different European countries. In order to assess the impact on the receiving water bodies, gene abundances in the latter were also analysed.Six out of the nine ARGs analysed were detected in all effluent and river water samples. Among the quantified genes, intI1 and sul1 were the most abundant. Our results demonstrate that European WWTP contribute to the enrichment of the resistome in the receiving water bodies with the particular impact being dependent on the effluent load and local hydrological conditions. The ARGs concentrations in WWTP effluents were found to be inversely correlated to the number of implemented biological treatment steps, indicating a possible option for WWTP management.Furthermore, this study has identified bla(OXA-58) as a possible resistance gene for future studies investigating the impact of WWTPs on their receiving water. (C) 2019 The Authors. Published by Elsevier Ltd.