Biodistribution of 99mTc-C60(OH)x in Sprague–Dawley rats after intratracheal instillation
Biodistribution of 99mTc-C60(OH)x in Sprague–Dawley rats after intratracheal instillation
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DOI：
10.1016/j.carbon.2007.04.030
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发表时间：
2007-08
期刊：
影响因子：
10.9
通讯作者：
Jing-ying Xu;Qingnuan Li;Jungang Li;Tiecheng Ran;Sheng-wei Wu;Weimin Song;Shao-liang Chen;Wenxin Li
中科院分区：
文献类型：
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作者：
Jing-ying Xu;Qingnuan Li;Jungang Li;Tiecheng Ran;Sheng-wei Wu;Weimin Song;Shao-liang Chen;Wenxin Li
越来越多的研究致力于研究碳纳米材料的呼吸道毒性，但迄今为止，关于这些纳米材料在吸入或气管内滴注后的生物分布的信息还知之甚少。合成了富勒烯C60（OH）x（x= 22，24）的多羟基衍生物，并用99 mTc标记。应用单光子发射计算机断层显像和γ射线计数技术，研究了99 mTc-C60（OH）在SD大鼠体内的生物分布。结果表明，99 mTc-C_（60）（OH）_x在肺中的滞留量最高，主要分布在肝、骨和脾中，脑中未见摄取。在肺中的长期保留和从血液中的非常快速的清除揭示了穿透肺泡-毛细血管屏障的瞬时特征。99 mTc-C_（60）（OH）_x的尺寸、分布以及体内聚集性可能影响其穿透肺泡-毛细血管屏障的能力和动力学过程。研究结果为进一步研究碳纳米材料的呼吸毒性提供了指导。
An increasing number of studies have been devoted to studying the respiratory toxicity of carbon nanomaterials, but little information is known, thus far, on the biodistribution of these nanomaterials after inhalation or intratracheal instillation. We synthesized and labeled a polyhydroxylated derivative of fullerene C60(OH)x(x=22,24) with99mTc. With single photon emission computed tomography imaging and 〈gamma〉-ray counting techniques, the biodistribution of99mTc-C60(OH)xin Sprague–Dawley rats after intratracheal instillation was studied. It was found that, besides the highest retention in the lung,99mTc-C60(OH)xis distributed mainly in the liver, bone, and spleen, with no uptake found in brain. The long-term retention in the lung and the very fast clearance from the blood revealed a transient characteristic of penetrating the alveolar-capillary barrier. The dimensions and size distribution, as well as in vivo aggregation of99mTc-C60(OH)x, may affect the capability and kinetic process of penetrating the alveolar-capillary barrier. The results provide guidance for further study of the respiratory toxicity of carbon nanomaterials.