CD2 expression on murine intestinal intraepithelial lymphocytes is bimodal and defines proliferative capacity.
CD2 expression on murine intestinal intraepithelial lymphocytes is bimodal and defines proliferative capacity.
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DOI：
10.1093/intimm/5.6.665
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发表时间：
1993-06
影响因子：
4.4
通讯作者：
N. Van Houten;P. Mixter;J. Wolfe;R. Budd
中科院分区：
文献类型：
--
作者：
N. Van Houten;P. Mixter;J. Wolfe;R. Budd
CD2分子在几乎所有小鼠淋巴细胞上正常表达，并通过抗原受体（TCR）共同刺激T细胞活化。在肠上皮内淋巴细胞（IEL）中发现了自然发生的CD2双峰表达T淋巴细胞群。TCR α β + IEL含有CD2-和CD2+细胞的比例大致相等，而TCR γ δ + IEL以CD2-细胞为主。IEL对抗cd3单抗或PMA +离子霉素与CD2表达共分离刺激的增殖反应；CD2+亚群在这些条件下增殖旺盛，而CD2-亚群则反应较弱。响应的CD2+ IEL包含TCR α β +和TCR γ δ +细胞。然而，用抗cd3单抗激活CD2- IEL只导致TCR γ δ + IEL的扩增，而用PMA +离子霉素激活并不促进TCR α β +或TCR γ δ + IEL的扩增。这些发现与自身免疫性lpr小鼠的观察结果相似，在lpr小鼠中，大量缺乏CD2表达的外周TCR α β + CD4-CD8- T细胞也对有丝分裂刺激反应低下。来自lpr小鼠的CD2- TCR α β + IEL以及CD2- T细胞的明显能量表明，TCR α β + T淋巴细胞上CD2的缺失与无反应性共分离。
The CD2 molecule is normally expressed on nearly all murine lymphocytes, and is co-stimulatory in T cell activation via the antigen receptor (TCR). A naturally occurring T lymphocyte population that is bimodal for CD2 expression was found in the intestinal intraepithelial lymphocytes (IEL). TCR alpha beta + IEL contain CD2- and CD2+ cells of approximately equal proportion, while TCR gamma delta + IEL are predominantly CD2-. The proliferative response of IEL to stimulation with an anti-CD3 mAb or with PMA plus ionomycin co-segregated with CD2 expression; the CD2+ subset proliferated vigorously under these conditions while the CD2- subset was much less responsive. The responding CD2+ IEL contained both TCR alpha beta + and TCR gamma delta + cells. However, activation of the CD2- IEL with anti-CD3 mAb resulted in only the expansion of TCR gamma delta + IEL, while activation with PMA plus ionomycin did not promote expansion of either the TCR alpha beta + or the TCR gamma delta + IEL. These findings parallel observations in the autoimmune lpr mouse, where massive numbers of peripheral TCR alpha beta + CD4-CD8- T cells that lack CD2 expression are also hyporesponsive to mitogenic stimulation. The apparent anergy of CD2- TCR alpha beta + IEL, as well as CD2- T cells from lpr mice, demonstrates that the absence of CD2 on TCR alpha beta + T lymphocytes co-segregates with nonresponsiveness.