Physical mappings of chloroplast DNA from liverwort Marchantia polymorpha L. cell suspension cultures-猫眼课题宝

地钱地钱细胞悬浮培养物叶绿体 DNA 的物理图谱

DOI：

10.1007/bf00326047

发表时间：

1983

期刊：

Molecular and General Genetics MGG

影响因子：

通讯作者：

中科院分区：

文献类型：

作者：

K. Ohyama;Y. Yamano;H. Fukuzawa;T. Komano;H. Yamagishi;Sin;M. Sugiura

Physical maps of liverwortMarchantia polymorphachloroplast DNA were constructed with restriction endonucleases,BamHI,SmaI,KpnI, andXhoI. The molecular size, calculated from the sum of the restriction fragments, is 121.0±1.0 kilobase (kb) pairs. This value is in good agreement with that of 118.7±2.0 kb pairs obtained from contour length measurements of the chloroplast DNA electron micrographs. The physical map indicates that the chloroplast DNA contains two copies of inverted repeats. The length of the inverted repeat is at most 11.7 kb pairs. Southern hybridization analysis indicates that two sets of the chloroplast ribosomal RNA genes are located in the inverted repeat regions. The site of the ribulose bisphosphate (RuBP) carboxylase (large subunit) gene was also determined on aBamHI fragment, Ba5, by using32P-labeled DNA fragments containing the tobacco chloroplast RuBP carboxylase gene as a probe.