Simultaneous analysis of N-nornuciferine, O-nornuciferine, nuciferine, and roemerine in leaves of Nelumbo nucifera Gaertn by high-performance liquid chromatography-photodiode array detection-electrospray mass spectrometry
Simultaneous analysis of N-nornuciferine, O-nornuciferine, nuciferine, and roemerine in leaves of Nelumbo nucifera Gaertn by high-performance liquid chromatography-photodiode array detection-electrospray mass spectrometry
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DOI：
10.1016/j.aca.2005.01.066
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发表时间：
2005-05-04
影响因子：
6.2
通讯作者：
Yao, SZ
中科院分区：
文献类型：
--
作者：
Luo, XB;Chen, B;Yao, SZ
建立了高效液相色谱-光电二极管阵列检测-电喷雾质谱联用分析莲叶中N-去荷叶碱、O-去荷叶碱、荷叶碱和罗梅碱的新方法。采用岛津VP-ODS柱，以0.1%三乙胺水溶液-乙腈为梯度溶剂体系。N-去甲牡丹碱、O-去甲牡丹碱、荷叶碱和罗梅碱均用标准化合物和MS光谱进行了鉴定。用差示扫描量热法测定了这些化合物的含量。这些化合物的检出限在30-90pg范围内，一般获得了大约三个数量级的浓度线性。(C)2005 Elsevier B.V.保留所有权利。
A novel method has been developed for analysis of N-nornuciferine, O-nomuciferine, nuciferine, and roemerine in leaves of Nelumbo nucifera Gaertn by using high-performance liquid chromatography (HPLC)-photodiode array detection (DAD)-electrospray mass spectrometry (ESI-MS). The method was carried out by using a Shimadzu VP-ODS column with a gradient solvent system of 0.1% triethylamine aqueous solution-acetonitrile. N-Nomuciferine, O-nomuciferine, nuciferine, and roemerine were identified with authentic standard compounds and with MS-spectra. The contents of these compounds were measured by employing DAD. Linearity of around three orders in the magnitude of concentration was generally obtained and limits of detection for these compounds were in the range of 30-90 pg. (c) 2005 Elsevier B.V. All rights reserved.