Poly(dimethylsiloxane) microchip electrophoresis with contactless conductivity detection for measurement of chemical warfare agent degradation products
Poly(dimethylsiloxane) microchip electrophoresis with contactless conductivity detection for measurement of chemical warfare agent degradation products
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DOI：
10.1080/00032710701792943
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发表时间：
2008-01-01
影响因子：
2
通讯作者：
Rogers, Kim R.
中科院分区：
文献类型：
--
作者：
Ding, Yongsheng;Garcia, Carlos D.;Rogers, Kim R.
报道了一种具有非接触电导率检测的聚二甲基硅氧烷（PDMS）微流控装置用于测定有机膦酸盐神经毒剂降解产物的适用性。采用微芯片毛细管电泳法对5种烷基甲基膦酸、异丙基甲基膦酸（IMPA）、蒎酰甲基膦酸（PMPA）、o -乙基- n、n -二甲基磷酰胺（EDPA）、乙基甲基膦酸（EMPA）和甲基膦酸（MPA）（沙林、索曼、图巴和VX神经毒剂的降解产物）进行了分析。实验条件的分离和检测过程进行了优化，以产生良好的分离和高灵敏度。在最佳条件下，分析在不到2分钟内完成。浓度与峰高呈线性关系，检出限在1.3 ~ 4.5mg/l范围内，峰高精密度值在3.4 ~ 6.1% RSD范围内。该方法对自然（湖泊和自来水）水样的适用性也进行了论证。与传统的分析方法相比，该系统具有成本效益高、操作简单、便携性好、样品消耗少等优点，有望实现对化学战剂降解产物的现场监测。
The applicability of a poly(dimethylsiloxane) (PDMS) microfluidic device with contactless conductivity detection for the determination of organophosphonate nerve agent degradation products is reported. Five alkyl methylphosphonic acids, isopropyl methylphosphonic acid (IMPA), pinacolyl methylphosphonic acid (PMPA), O-ethyl-N,N-dimethyl phosphoramidate (EDPA), ethyl methylphosphonic acid (EMPA), and methylphosphonic acid (MPA), (degradation products of Sarin, Soman, Tuban and VX nerve agents) were analyzed by microchip capillary electrophoresis. Experimental conditions for the separation and detection processes have been optimized to yield well-defined separation and high sensitivity. Under optimal conditions, analyses were completed in less than 2min. Linear relations between concentration and peak heights were obtained with detection limits in the 1.3-4.5mg/l range and precision values for the peak heights were in the range of 3.4-6.1% RSD. Applicability of this method for natural (lake and tap) water samples was also demonstrated. Compared to conventional analytical methods, this miniaturized system offers promise for on-site monitoring of degradation products of chemical warfare agents, with advantages of cost effective construction, simple operation, portability, and minimum sample consumption.