The First Structure of a Mycobacteriophage, the Mycobacterium abscessus subsp bolletii Phage Araucaria
The First Structure of a Mycobacteriophage, the Mycobacterium abscessus subsp bolletii Phage Araucaria
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DOI：
10.1128/jvi.01209-13
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发表时间：
2013-07-01
影响因子：
5.4
通讯作者：
Cambillau, Christian
中科院分区：
文献类型：
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作者：
Sassi, Mohamed;Bebeacua, Cecilia;Cambillau, Christian
蜡质分枝杆菌细胞壁的独特特性使人们对其噬菌体的特殊结构特征产生了疑问。目前还没有任何分支杆菌噬菌体的结构可用，尽管到目前为止已经描述了类似于3500的结构。为了填补这一空白，我们开始了对脓肿分枝杆菌亚种噬菌体的基因组和结构的研究。Bolletii，脓肿分枝杆菌组的成员。这种机会性病原体是肺部疾病患者呼吸道感染的原因，尤其是囊性纤维化。脓肿支原体亚种从呼吸道标本中分离到博莱蒂菌，并在培养物中观察到噬菌体。我们在这里报告了脓肿分支杆菌亚种的基因组注释和特征。以及首次用单粒子电子显微镜重建了整个病毒粒子。该菌属于剑毒科，拥有一个-kb的基因组，包含89个开放阅读框(ORF)，其中27个可以确定地进行注释。虽然它的衣壳和连接器与革兰氏阴性(Gram(-))或革兰氏阴性(Gram(+))细菌的几个噬菌体非常相似，但它最显著的特征是其螺旋尾巴上装饰着放射状的尖刺，可能是宿主黏附装置，噬菌体的名字是根据这个装置选择的。它的宿主吸附装置在尾端，组装了在与蛋白质受体结合的噬菌体中观察到的特征，例如噬菌体SPP1。综上所述，这些结果表明，Araucaria可能通过与细胞壁糖和蛋白质黏附的机制感染其分枝杆菌宿主，这一特征仍有待进一步探索。
The unique characteristics of the waxy mycobacterial cell wall raise questions about specific structural features of their bacteriophages. No structure of any mycobacteriophage is available, although similar to 3,500 have been described to date. To fill this gap, we embarked in a genomic and structural study of a bacteriophage from Mycobacterium abscessus subsp. bolletii, a member of the Mycobacterium abscessus group. This opportunistic pathogen is responsible for respiratory tract infections in patients with lung disorders, particularly cystic fibrosis. M. abscessus subsp. bolletii was isolated from respiratory tract specimens, and bacteriophages were observed in the cultures. We report here the genome annotation and characterization of the M. abscessus subsp. bolletii prophage Araucaria, as well as the first single-particle electron microscopy reconstruction of the whole virion. Araucaria belongs to Siphoviridae and possesses a 64-kb genome containing 89 open reading frames (ORFs), among which 27 could be annotated with certainty. Although its capsid and connector share close similarity with those of several phages from Gram-negative (Gram(-)) or Gram(+) bacteria, its most distinctive characteristic is the helical tail decorated by radial spikes, possibly host adhesion devices, according to which the phage name was chosen. Its host adsorption device, at the tail tip, assembles features observed in phages binding to protein receptors, such as phage SPP1. All together, these results suggest that Araucaria may infect its mycobacterial host using a mechanism involving adhesion to cell wall saccharides and protein, a feature that remains to be further explored.