THE WRINKLED-SEED CHARACTER OF PEA DESCRIBED BY MENDEL IS CAUSED BY A TRANSPOSON-LIKE INSERTION IN A GENE ENCODING STARCH-BRANCHING ENZYME
THE WRINKLED-SEED CHARACTER OF PEA DESCRIBED BY MENDEL IS CAUSED BY A TRANSPOSON-LIKE INSERTION IN A GENE ENCODING STARCH-BRANCHING ENZYME
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DOI：
10.1016/0092-8674(90)90721-p
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发表时间：
1990-01-12
期刊：
影响因子：
64.5
通讯作者：
MARTIN, C
中科院分区：
文献类型：
--
作者：
BHATTACHARYYA, MK;SMITH, AM;MARTIN, C
我们描述了豌豆（Pisum sativum L.） r （rugosus）位点的克隆，它决定了种子是圆的还是皱的。皱粒（rr）种子缺乏淀粉分支酶（SBEI）的一种异构体，这种异构体存在于圆粒（rr或rr）种子中。SBEI基因在近等基因RR系和RR系之间的主要多态性与r-座的共分离率为100%，表明SBEI基因位于r座。在rr胚胎中产生了异常的SBEI转录本。在rr系中，SBEI基因被一个0.8 kb的插入打断，这与玉米中的Ac/Ds转座元件家族非常相似。不能产生SBEI对种子中淀粉、脂质和蛋白质的生物合成有复杂的代谢后果。
We describe the cloning of the r (rugosus) locus of pea (Pisum sativum L.), which determines whether the seed is round or wrinkled. Wrinkled (rr) seeds lack one isoform of starch-branching enzyme (SBEI), present in round (RR or Rr) seeds. A major polymorphism in the SBEI gene between near-isogenic RR and rr lines shows 100% cosegregation with the r-locus, establishing that the SBEI gene is at the r locus. An aberrant transcript for SBEI is produced in rr embryos. In rr lines the SBEI gene is interrupted by a 0.8 kb insertion that is very similar to the Ac/Ds family of transposable elements from maize. Failure to produce SBEI has complex metabolic consequences on starch, lipid, and protein biosynthesis in the seed.