Sperm protein 17 influences the tissue-specific malignancy of clear cell adenocarcinoma in human epithelial ovarian cancer
Sperm protein 17 influences the tissue-specific malignancy of clear cell adenocarcinoma in human epithelial ovarian cancer
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DOI：
10.1111/j.1525-1438.2007.00815.x
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发表时间：
2007-03-01
影响因子：
4.8
通讯作者：
Minegishi, T.
中科院分区：
文献类型：
--
作者：
Nakazato, T.;Kanuma, T.;Minegishi, T.
卵巢透明细胞腺癌由于化疗耐药和早期淋巴结转移，预后较差。它还会导致子宫内膜异位症，是日本第二常见的卵巢癌类型。卵巢浆液性腺癌是日本另一种常见的上皮性癌组织亚型，对化疗高度敏感。在目前的研究中，我们检测了这些类型的卵巢癌中基因的差异表达，并试图分析它们的功能，特别是当它们与化疗耐药有关时。我们用差异显示法比较了卵巢透明细胞癌和浆液性腺癌。我们发现精子蛋白17(SP17)是与透明细胞癌化疗耐药相关的候选基因。实时定量聚合酶链式反应证实其差异表达。由于SP17基因在卵巢癌中的作用尚不清楚，我们检测了针对SP17基因的小干扰RNA对ES-2卵巢癌细胞对紫杉醇的耐药性和增殖的影响，紫杉醇是目前治疗卵巢癌最有效的药物。我们发现，这种治疗降低了这些细胞对紫杉醇的化疗耐药性。我们的结果有力地表明，SP17在透明细胞癌对化疗的抵抗中起作用，而不影响其增殖能力。
Clear cell adenocarcinoma of the ovary has a poor prognosis due to chemoresistance and early metastasis to the lymph nodes. It also can result in endometriosis and is the second most frequent type of ovarian cancer in Japan. Serous adenocarcinoma of the ovary is another common epithelial cancer tissue subtype in Japan, and it is highly sensitive to chemotherapy. In the current study, we examined the differential expression of genes in these types of ovarian cancer and tried to analyze their functions, especially as they relate to chemoresistance. We used differential display to compare clear cell carcinoma and serous adenocarcinoma of the ovary. We identified sperm protein 17 (SP17) as a candidate gene related to the chemoresistance of clear cell carcinoma. Its differential expression was confirmed by real-time polymerase chain reaction. Because the function of the SP17 gene in ovarian cancer is not known, we examined the effect of small interfering RNA targeting the SP17 gene on the chemoresistance and proliferation of ES-2 ovarian cancer cells to paclitaxel, currently the most effective treatment for ovarian cancer. We found that this treatment decreased the chemoresistance of these cells to paclitaxel. Our results strongly suggest that SP17 plays a role in the resistance of clear cell carcinoma to chemotherapy without influencing their ability to proliferate.