Migration and function of a glial subtype in the vertebrate peripheral nervous system
Migration and function of a glial subtype in the vertebrate peripheral nervous system
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DOI：
10.1016/s0896-6273(02)00683-9
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发表时间：
2002-05-16
期刊：
影响因子：
16.2
通讯作者：
Nüsslein-Volhard, C
中科院分区：
文献类型：
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作者：
Gilmour, DT;Maischein, HM;Nüsslein-Volhard, C
神经胶质-轴突相互作用对于神经系统的发育和功能是必不可少的。我们结合联合收割机在体内成像和遗传学，以解决机制，这些细胞的迁移是协调在胚胎发育。使用稳定的转基因系，我们已经遵循了迁移的一个子集的神经胶质细胞和它们的目标轴突在活斑马鱼胚胎。这些细胞在早期阶段合并，并在整个迁移过程中保持耦合，轴突显然为神经胶质细胞寻路。突变体分析表明，轴突提供了指导性的线索，足以控制神经胶质的指导。此外，转录因子Sox 10/cls的突变解除了轴突和神经胶质的迁移。最后，这种胶质细胞亚型的基因消融揭示了神经成束的重要作用。
Glia-axon interactions are essential for the development and function of the nervous system. We combine in vivo imaging and genetics to address the mechanism by which the migration of these cells is coordinated during embryonic development. Using stable transgenic lines, we have followed the migration of one subset of glial cells and their target axons in living zebrafish embryos. These cells coalesce at an early stage and remain coupled throughout migration, with axons apparently pathfinding for glia. Mutant analysis demonstrates that axons provide instructive cues that are sufficient to control glial guidance. Furthermore, mutations in the transcription factor Sox10/cls uncouple the migration of axons and glia. Finally, genetic ablation of this glial subtype reveals an essential role in nerve fasciculation.