Demonstration of two stable potential states in the squid giant axon under tetraethylammonium chloride.
Demonstration of two stable potential states in the squid giant axon under tetraethylammonium chloride.
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DOI：
10.1085/jgp.40.6.859
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发表时间：
1957-07-20
期刊：
影响因子：
--
通讯作者：
HAGIWAR AS
中科院分区：
文献类型：
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作者：
TASAKI I;HAGIWAR AS
1.将四乙基氯化铵（TEA）细胞内注射到鱿鱼的巨大轴突中，可以延长动作电位的持续时间，而不改变静息电位（图3）。延长有时是100倍或更多。2.用TEA处理的巨大轴突的动作电位具有初始峰值，随后是平台（图3）。在平台期的膜电阻实际上是正常的（图4）。在接近动作电位结束时，膜电阻明显增加（图5D和图6，右）。3.在用TEA处理的鱿鱼巨轴突中证实了动作电位消除的现象（图7）。动作电位在早期消除后，不存在不应期（图8）。4.通过电压钳方法，研究了正常鱿鱼轴突以及TEA处理的轴突上的电压-电流关系（图1和图2）。（第9和10段）。5.通过用两个电压阶跃钳制膜电位来证明膜的稳定状态的存在（图11）。实验证据表明，在一个“不稳定”的状态下，膜电导是不唯一的膜电位决定。6.在用TEA处理的轴突中研究了低钠水的作用（图12）。7.强调了鱿鱼轴突在TEA下的动作电位与脊椎动物心肌的动作电位之间的相似性。实验结果被解释为支持膜中存在两种稳定状态的观点。动作电位的启动和消除被解释为两种状态之间的过渡。
1. Intracellular injection of tetraethylammonium chloride (TEA) into a giant axon of the squid prolongs the duration of the action potential without changing the resting potential (Fig. 3). The prolongation is sometimes 100-fold or more. 2. The action potential of a giant axon treated with TEA has an initial peak followed by a plateau (Fig. 3). The membrane resistance during the plateau is practically normal (Fig. 4). Near the end of the action potential, there is an apparent increase in the membrane resistance (Fig. 5D and Fig. 6, right). 3. The phenomenon of abolition of action potentials was demonstrated in the squid giant axon treated with TEA (Fig. 7). Following an action potential abolished in its early phase, there is no refractoriness (Fig. 8). 4. By the method of voltage clamp, the voltage-current relation was investigated on normal squid axons as well as on axons treated with TEA (Figs. 9 and 10). 5. The presence of stable states of the membrane was demonstrated by clamping the membrane potential with two voltage steps (Fig. 11). Experimental evidence was presented showing that, in an "unstable" state, the membrane conductance is not uniquely determined by the membrane potential. 6. The effect of low sodium water was investigated in the axon treated with TEA (Fig. 12). 7. The similarity between the action potential of a squid axon under TEA and that of the vertebrate cardiac muscle was stressed. The experimental results were interpreted as supporting the view that there are two stable states in the membrane. Initiation and abolition of an action potential were explained as transitions between the two states.