Correlation between the regulation of intestinal bacteriophages by green tea polyphenols and the flora diversity in SPF mice
Correlation between the regulation of intestinal bacteriophages by green tea polyphenols and the flora diversity in SPF mice
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绿茶多酚调节SPF小鼠肠道噬菌体与菌群多样性的相关性
DOI：
10.1039/d1fo03694g
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发表时间：
2022-02-04
期刊：
影响因子：
6.1
通讯作者：
Fang, Xiang
中科院分区：
文献类型：
--
作者：
Dong, Sashuang;Xin, Zilong;Fang, Xiang
绿色茶多酚（GTP）在塑造肠道微生物组中起着重要作用，肠道微生物组包括一系列密集定植的微生物，包括噬菌体。以前的研究集中在GTP对肠道微生物群中细菌的影响上。然而，关于GTP在健康肠道的噬菌体组成中的作用知之甚少。在这项研究中，SPF雄性C57 BL/6 J小鼠被分为无多酚饮食组和茶多酚饮食组，其中饮用水中添加0.1%GTP，持续28天。对小鼠粪便样品进行病毒样颗粒制备物的超深度宏基因组测序和细菌16 S rRNA测序。然后比较各组之间肠道细菌组、噬菌体和细菌-噬菌体相关性的变化。结果显示，在GTP暴露后，厚壁菌门的丰度、拟杆菌门的显著减少和F/B的比率的显著增加。GTP改变了双歧杆菌（相对丰度> 1.00%）和阿克曼氏菌（相对丰度> 1.00%）的丰度（相对丰度调节率为89.78%，相对丰度上调0.89%）。经GTP处理后，粪杆菌的丰度（调节率为60.17%）增加了24.38%。GTP还改变了沙门氏菌噬菌体的丰度（调节率为98.64%，丰度上调3.16%）和戈登氏菌噬菌体益力多的丰度（调节率为99.99%，丰度下调5.44%）。显著提高了肠道的溶解性，降低了29.22%的温度。优势菌（相对丰度>1.00%）双歧杆菌和杜氏杆菌与粪杆菌噬菌体呈显著负相关，与乳杆菌原噬菌体呈显著正相关。暴露于GTP积极促进SPF小鼠肠道噬菌体群落和微生物群落相互作用网络的变化。这些发现强调了“有益的”噬菌体-细菌关系的重要性，并揭示了GTP通过肠道噬菌体群落调节肠道植物群的潜在机制。
Green tea polyphenols (GTP) play an important role in shaping the gut microbiome, comprising a range of densely colonizing microorganisms, including bacteriophages. Previous studies focused on the effect of GTP on the bacteria in the gut microbiota. However, little is known about the role of GTP in the bacteriophage composition of healthy intestines. In this study, SPF male C57BL/6J mice were divided into a polyphenol-free diet group and a tea polyphenol diet group where drinking water was supplemented with 0.1% GTP for 28 days. The ultra-deep metagenomic sequencing of virus-like particle preparations and bacterial 16S rRNA sequencing were performed on mouse stool samples. Changes in the gut bacteriome, bacteriophages, and bacterial-bacteriophage correlations were then compared between the groups. The results revealed an abundance of Firmicutes, a significant decrease in Bacteroidetes, and a significant increase in the ratio of F/B after GTP exposure. The GTP altered the abundance (relative abundance > 1.00%) of Bifidobacterium (regulation rate of 89.78% and the abundance up-regulated by 0.89%) and Akkermansia (regulation rate of 99.70% and the abundance down-regulated by 1.77%). The abundance of Faecalibaculum (regulation rate of 60.17%) increased by 24.38% following GTP treatment. The GTP also altered the abundance of Salmonella phage (regulation rate of 98.64% and the abundance up-regulated by 3.16%) and that of Gordonia_phage_Yakult (regulation rate of 99.99% and the abundance down-regulated by 5.44%). It significantly increased the intestine's lytic phages and reduced the temperate phages by 29.22%. The dominant microorganisms (relative abundance >1.00%) of Bifidobacterium and Dubosiella had a significantly negative relationship with the Faecalibacterium phage and a significantly positive relationship with the Lactobacillus prophage. Exposure to GTP positively promoted changes in the gut bacteriophage community and interaction network in the microbial community of the SPF mice. These findings highlight the importance of "profitable" bacteriophage-bacteria relationships and reveal a potential mechanism of GTP towards the regulation of intestinal flora via intestinal phage communities.