Development of Ferroelectric P(VDF-TrFE) Microparticles for Ultrasound-Driven Cancer Cell Killing.
Development of Ferroelectric P(VDF-TrFE) Microparticles for Ultrasound-Driven Cancer Cell Killing.
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DOI：
10.1021/acsami.3c13410
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发表时间：
2023-11
影响因子：
9.5
通讯作者：
Zulmari Silva Pedraza;Yizhan Wang;Corey Carlos;Zhongmin Tang;Jun Li;Weibo Cai;Xudong Wang
中科院分区：
文献类型：
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作者：
Zulmari Silva Pedraza;Yizhan Wang;Corey Carlos;Zhongmin Tang;Jun Li;Weibo Cai;Xudong Wang
目前的乳腺癌治疗涉及积极和侵入性的方法，为新的治疗方法的出现留下了空间。在这项工作中，我们探讨了使用压电[P（VDF-TrFE）]微粒（MP）作为诱导4 T1乳腺癌细胞不可逆电穿孔（IRE）的来源的可能性。我们详细介绍了MP的形成机制和尺寸控制以及随后的表征，证实了压电β相的存在。通过超声搅拌实现MP的必要压电输出的产生。我们确认的主要因素的IRE对4 T1乳腺癌细胞的影响是从MP产生的局部电场，通过使用共聚焦成像和alamarBlue测定。结果显示细胞活力降低52.6%，表明MP处理可有助于减少活癌细胞。所提出的超声刺激P（VDF-TrFE）MP的方法可以提供更良性的癌症治疗方法。
Current breast cancer treatments involve aggressive and invasive methods, leaving room for new therapeutic approaches to emerge. In this work, we explore the possibility of using piezoelectric [P(VDF-TrFE)] microparticles (MPs) as a source of inducing irreversible electroporation (IRE) of 4T1 breast cancer cells. We detail the MP formation mechanism and size control and subsequent characterizations of the as-synthesized MPs which confirms the presence of piezoelectric β-phase. Production of the necessary piezoelectric output of the MPs is achieved by ultrasound agitation. We confirm the primary factor of the IRE effect on 4T1 breast cancer cells to be the local electric field produced from the MPs by using confocal imaging and an alamarBlue assay. The results show a 52.6% reduction in cell viability, indicating that the MP treatment can contribute to a reduction of live cancer cells. The proposed method of ultrasound-stimulated P(VDF-TrFE) MPs may offer a more benign cancer treatment approach.