Decreased concentration of IL-35 in plasma of patients with asthma and COPD
Decreased concentration of IL-35 in plasma of patients with asthma and COPD
复制标题
哮喘和慢性阻塞性肺病患者血浆中 IL-35 浓度降低
DOI：
10.12932/ap0403.32.3.2014
复制
发表时间：
2014-09-01
影响因子：
5
通讯作者：
Xie, Jungang
中科院分区：
文献类型：
--
作者：
Chen, Chen;Deng, Yanhan;Xie, Jungang
背景：IL-35已被发现参与人类许多炎症性疾病，但其在哮喘和慢性阻塞性肺疾病（COPD）中的作用尚不清楚。哮喘和COPD患者血浆IL-35水平需要检测。目的：研究新诊断哮喘和慢性阻塞性肺病患者及对照组的血浆IL-35水平，探讨肺功能、年龄、性别、吸烟史与哮喘和慢性阻塞性肺病患者血浆IL-35水平的相关性。方法：采集新诊断哮喘患者（男性44,12例，年龄33.75 +/- 8.94）、新诊断COPD患者（男性36,36例，年龄68.03 +/- 8.94）和健康对照组（男性23,9例，年龄30.06 +/- 7.50）的血液样本。采用酶联免疫吸附法测定血浆中IL-35水平。结果：哮喘患者IL-35的中位值和范围为118.55 pg/ml（范围74.43相似于1767.22 pg/ml）， COPD患者为136.09 pg/ml（范围62.54相似于697.49 pg/ml），对照组为275.86 pg/ml（范围26.11相似于4766.20 pg/ml）。IL-35≥150 pg/ml的哮喘患者血浆IL-35水平与FEV1%、FVC%呈正相关。血浆IL-35值大于150 pg/ml的COPD患者血浆IL-35与FVC%之间也存在正相关。结论：这些发现提示IL-35很可能参与了Th2和Th17介导的哮喘和COPD炎症过程。其在人类COPD和哮喘发病机制中的作用及其在这些疾病中的治疗价值有待进一步研究。
Background: IL-35 has been found to be involved in many inflammatory diseases in humans but its role in asthma and chronic obstructive pulmonary disease (COPD) is not clear. The plasma level of IL-35 in patients with asthma and COPD needs to be measured.Objective: The aim of this study was to examine the plasma concentrations of IL-35 in newly diagnosed asthmatic and COPD patients and control subjects and investigate correlations of lung function, age, sex, smoking history with the levels of IL-35 in plasma in these diseases.Methods: Blood samples were collected from patients with newly diagnosed asthma (44, 12 males, aged 33.75 +/- 8.94), newly diagnosed COPD (36, 36 males, aged 68.03 +/- 8.94), and healthy control groups (23, 9 males, aged 30.06 +/- 7.50). We determined the IL-35 levels in plasma by enzyme-linked immunosorbent assay.Result: The median and the range of values for IL-35 were 118.55 pg/ml (range 74.43 similar to 1767.22 pg/ml) in patients with asthma, 136.09 pg/ml (range 62.54 similar to 697.49 pg/ml) in patients with COPD and 275.86 pg/ml (range 26.11 similar to 4766.20 pg/ml) in control subjects. The levels of IL-35 in plasma showed a positive correlation with FEV1% and FVC% in asthmatic patients whose plasma IL-35 values were over 150 pg/ml. A positive correlation was also found between plasma IL-35 and FVC% in COPD patients whose plasma IL-35 values were over 150 pg/ml.Conclusions: These findings suggest that IL-35 may very probably be involved in the Th2 and Th17 mediated inflammation process of asthma and COPD. Its role in the mechanisms of COPD and asthma in human beings, as well as its therapeutic value in these diseases, need further investigation.