Phosphoproteomic basis of neuroplasticity in the antennal lobes influences the olfactory differences between Apis mellifera and Apis cerana honeybees.
Phosphoproteomic basis of neuroplasticity in the antennal lobes influences the olfactory differences between Apis mellifera and Apis cerana honeybees.
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DOI：
10.1016/j.jprot.2021.104413
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发表时间：
2021-10
影响因子：
3.3
通讯作者：
Lifeng Meng;S. Z. Altaye;M. Feng;Han Hu;Bin Han;Jianke Li
中科院分区：
文献类型：
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作者：
Lifeng Meng;S. Z. Altaye;M. Feng;Han Hu;Bin Han;Jianke Li
蜜蜂属A种。意大利人和A。Cerana在嗅觉驱动的行为和外周嗅觉系统方面进化出了实质性的差异。然而，关于中枢神经系统调节这些嗅觉差异的知识是有限的。我们比较了触角叶(ALS，初级嗅神经纤维)OFA的磷蛋白质组。意大利人和A。Cerana，并从1265个磷酸蛋白中鉴定出2812个携带2971个磷酸位点的磷酸肽。其中76%的磷蛋白是两种生物共有的，主要是突触前和细胞骨架成分，并参与信号转导和神经递质的分泌。这一发现表明蛋白质磷酸化在调节肌萎缩侧索硬化症的信号转导中起着基础作用。在蛋白质代谢中重要的mTOR信号通路、吞噬小体通路和自噬通路被丰富，这表明肾小球的可塑性和嗅觉加工是通过这些通路被强烈的磷酸化调节的。与A相比。与蛋白质代谢和转运相关的107个磷酸蛋白在INA中得到了唯一的表达。Cerana，表明依赖蛋白质合成的突触可塑性增强。在山区觅食地区，樱桃有助于处理更复杂的花卉气味线索。这一发现进一步得到了mTOR信号通路INA关键磷酸蛋白显著上调的支持。鹿角鱼。这些结果提供了对神经可塑性的磷酸蛋白质组学基础的洞察，该基础与蜜蜂在不同环境中的不同进化有关。意义为了适应自己的生态位，两个主要的蜜蜂物种，意大利蜜蜂和A。在嗅觉驱动的行为方面已经形成了显著的差异，但我们对中枢神经系统潜在调节的理解仍然有限。在这里，我们首次对A和A的触角叶(Als)进行了全面的磷酸蛋白质组学比较。意大利人和A。瑟琳娜。在ALS的信号转导和肾小球可塑性的调控中，这两个物种共享了很大比例的亚磷酸盐和磷蛋白。然而，与a.其中，与蛋白质代谢和转运相关的107个磷酸蛋白被鉴定为唯一的内毒素。CeranaALs，以及mTOR信号通路中的几个关键的磷酸蛋白也被发现上调INa。金龟子。这些结果表明，蛋白磷酸化增强了蛋白合成依赖的突触可塑性。在山区觅食地区，樱桃有助于处理更复杂的花卉气味线索。我们的研究结果为进一步研究蜜蜂和其他昆虫的神经生物学研究提供了有价值的见解，揭示了蜜蜂嗅觉行为的大脑调节机制。
The honeybee speciesA. melliferaandA. ceranahave evolved substantial differences in olfactory-driven behaviors and in peripheral olfactory systems. Knowledge of the central nervous system regulating these olfaction differences is limited, however. We compared the phosphoproteome of the antennal lobes (ALs, the primary olfactory neuropil) ofA. melliferaandA. cerana, and identified a total of 2812 phosphopeptides carrying 2971 phosphosites from 1265 phosphoproteins. Of these, 76% of the phosphoproteins were shared by both species, which were mainly presynapse and cytoskeleton components, and were involved in signal transduction and neurotransmitter secretion. This finding indicates the fundamental role of protein phosphorylation in regulating signal transduction in the ALs. The mTOR signaling pathway, the phagosome pathway, and the autophagy pathway, which are important in protein metabolism, were enriched, suggesting glomeruli plasticity and olfactory processing are intensively modulated by phosphorylation via these pathways. Compared withA. mellifera, 107 phosphoproteins associated with protein metabolism and transport were uniquely expressed inA. cerana, indicating the protein synthesis-dependent synaptic plasticity is enhanced inA. ceranato facilitate the processing of more complex floral odor clues in mountain foraging areas. This finding is further supported by the significantly upregulated key phosphoproteins of the mTOR signaling pathway inA. ceranaALs. These results provide insights into the phosphoproteomic basis of neuroplasticity that is coupled with the divergent evolution of bees in different environments.SignificanceTo adapt to their own ecological niche, the two major honeybee species,A. melliferaandA. cerana, have developed significant difference in olfactory-driven behaviors, but our understanding of the underlying regulation of the central nervous system is still limitate. Here we performed the first comprehensive phosphoproteomic comparison of antennal lobes (Als) betweenA. melliferaandA. cerena. A large proportion of the identified phosphosites and phosphoproteins were shared between the two species to serve as a core network in the regulation of signal transduction and glomeruli plasticity of ALs. However, compared withA. mellifera, 107 phosphoproteins associated with protein metabolism and transport were uniquely identified inA. ceranaALs, and also several key phosphoproteins in mTOR signaling pathway were found upregulated inA. cerana. These findings indicate protein phosphorylation enhanced the protein synthesis-dependent synaptic plasticity inA. ceranato facilitate the processing of more complex floral odor clues in mountain foraging areas. Our data provide a valuable insight into phosphoproteome-driven cerebral regulation of honeybee olfactory behaviors, which is potentially useful for further neurobiological investigation in both honeybees and other insects.