High cell density cultivation of recombinant yeasts and bacteria under non-pressurized and pressurized conditions in stirred tank bioreactors
High cell density cultivation of recombinant yeasts and bacteria under non-pressurized and pressurized conditions in stirred tank bioreactors
复制标题
DOI：
10.1016/j.jbiotec.2007.06.010
复制
发表时间：
2007-10-31
影响因子：
4.1
通讯作者：
Buechs, Jochen
中科院分区：
文献类型：
--
作者：
Knoll, Arnd;Bartsch, Stefan;Buechs, Jochen
本研究证明了加压搅拌槽生物反应器在好氧培养过程中用于氧传递增强的适用性。采用比功率输入和反应器压力作为过程变量。作为模式生物大肠杆菌，Arxula adeninivorans，酿酒酵母和谷氨酸棒杆菌培养到高细胞密度。通过应用大约特定的功率输入。48 kW·m ~（-3）的氧传递速率。将非加压搅拌罐生物反应器中的大肠杆菌培养物升高至0.51 mol L（-1）h（-1）的值。当施加高达10巴的反应器压力时，加压搅拌罐生物反应器的氧转移速率被提升至0.89 mol l（-1）h（-1）的值。非加压搅拌罐生物反应器能够支持细胞密度大于40 g l（-1）细胞干重（CDW）的E.而加压搅拌罐生物反应器能够支持细胞密度高达225 g l（-1）CDW的A. adeninivorans，89 g l（-1）CDW;酿酒酵母226 g l（-1）CDW; glutamicum和110 g l（-1）CDW。杆菌与文献数据相比，这些细胞密度中的一些是在搅拌罐生物反应器中微生物的高细胞密度培养中曾经达到的最高值。通过比较两种系统的比功率输入以及k（L）a值，证明了在工业搅拌槽生物反应器中，只有压力是用于氧传递增强的可缩放工具。此外，它表明，增加二氧化碳分压并没有显着抑制所研究的模式生物的生长。(c)2007 Elsevier B. V.保留所有权利。
This study demonstrates the applicability of pressurized stirred tank bioreactors for oxygen transfer enhancement in aerobic cultivation processes. The specific power input and the reactor pressure was employed as process variable. As model organism Escherichia coli, Arxula adeninivorans, Saccharomyces cerevisiae and Corynebacterium glutamicum were cultivated to high cell densities. By applying specific power inputs of approx. 48 kW m(-3) the oxygen transfer rate of a E. coli culture in the non-pressurized stirred tank bioreactor was lifted up to values of 0.51 mol l(-1) h(-1). When a reactor pressure up to 10 bar was applied, the oxygen transfer rate of a pressurized stirred tank bioreactor was lifted up to values of 0.89 mol l(-1) h(-1). The non-pressurized stirred tank bioreactor was able to support non-oxygen limited growth of cell densities of more than 40 g l(-1) cell dry weight (CDW) of E. coli, whereas the pressurized stirred tank bioreactor was able to support non-oxygen limited growth of cell densities up to 225 g l(-1) CDW of A. adeninivorans, 89 g l(-1) CDW of S. cerevisiae, 226 g l(-1) CDW of C. glutamicum and 110 g l(-1) CDW of E. coli. Compared to literature data, some of these cell densities are the highest values ever achieved in high cell density cultivation of microorganisms in stirred tank bioreactors. By comparing the specific power inputs as well as the k(L)a values of both systems, it is demonstrated that only the pressure is a scaleable tool for oxygen transfer enhancement in industrial stirred tank bioreactors. Furthermore, it was shown that increased carbon dioxide partial pressures did not remarkably inhibit the growth of the investigated model organisms. (c) 2007 Elsevier B.V. All rights reserved.