MK-801, AN OBS N-METHYL-D-ASPARTATE CHANNEL BLOCKER, DOES NOT IMPROVE THE FUNCTIONAL RECOVERY NOR SPINAL-CORD BLOOD-FLOW AFTER SPINAL-CORD COMPRESSION IN RATS
MK-801, AN OBS N-METHYL-D-ASPARTATE CHANNEL BLOCKER, DOES NOT IMPROVE THE FUNCTIONAL RECOVERY NOR SPINAL-CORD BLOOD-FLOW AFTER SPINAL-CORD COMPRESSION IN RATS
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DOI：
10.1111/j.1600-0404.1991.tb04964.x
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发表时间：
1991-10-01
影响因子：
3.5
通讯作者：
GERDIN, B
中科院分区：
文献类型：
--
作者：
HOLTZ, A;GERDIN, B
中枢神经系统受损后会局部释放兴奋性氨基酸，例如氨基酸。谷氨酸盐，据称可以增加已经缺氧的神经元的代谢需求，从而促进细胞死亡。为了研究 N-甲基-D-天冬氨酸 (NMDA) 受体介导的机制是否与脊髓损伤引起的损伤有关，20 只大鼠在 2.2 x 5 mm 大小的板上承受 35 g 负荷，对胸脊髓进行 5 分钟的压缩。一组动物给予剂量为 10 mg/kg b.w 的非竞争性 NMDA 通道阻断剂 MK-801，一组仅给予生理盐水。当用C-14-碘安替比林放射自显影技术测量脊髓血流(SCBF)时，在斜面上评估神经功能4天。创伤后一天，两组动物均处于截瘫状态，并且在斜面测试中表现出明显减小的容量角（与压缩前的约63度相比，约35度）。此后，两组的运动功能略有改善，但程度相似。第 4 天，两组的灰质和白质 SCBF 相似。结果表明，所用剂量的 MK 801 不能防止神经功能障碍的发生或脊髓受压后 SCBF 的减少。
Damage to the central nervous system is followed by local release of excitatory amino acids, e.g. glutamate, These have been claimed to increase the metabolic need of already hypoxic neurons, and thereby to promote cell death. To investigate whether N-methyl-D-aspartate (NMDA) receptor-mediated mechanisms are involved in the damage consequent to spinal cord injury, 20 rats were exposed to 5-min compression of the thoracic spinal cord produced with a load of 35 g on a 2.2 x 5 mm sized plate. One group of animals was given a noncompetitive NMDA channel blocker, MK-801, in a dose of 10 mg/kg b.w and one group saline alone. The neurologic function was evaluated on the inclined plane for 4 days when spinal cord blood flow (SCBF) was measured with the C-14-iodoantipyrine autoradiographic technique. One day after trauma the animals in both groups were paraparetic and exhibited a significantly decreased capacity angle at the inclined plane test (about 35-degrees compared with about 63-degrees before compression). Thereafter, the motor function improved slightly, but to a similar extent in the two groups. On Day 4, gray and white matter SCBF was similar in the two groups. The results indicate that MK 801 in the dose used does not prevent the development of neurologic dysfunction or the reduction in SCBF after spinal cord compression.