Terbium-Macrocycle Complexes as Chemical Sensors: Detection of an Aspirin Metabolite in Urine Using a Salicylurate-Specific Receptor Site
Terbium-Macrocycle Complexes as Chemical Sensors: Detection of an Aspirin Metabolite in Urine Using a Salicylurate-Specific Receptor Site
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DOI：
10.1021/ic1003066
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发表时间：
2010-05-17
影响因子：
4.6
通讯作者：
Ponce, Adrian
中科院分区：
文献类型：
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作者：
Esplin, Taran L.;Cable, Morgan L.;Ponce, Adrian
水杨脲 (SU) 是乙酰水杨酸（阿司匹林）在尿液中的主要代谢物，可用作监测阿司匹林药代动力学的指标以及阑尾炎、贫血和肝脏疾病的指标。目前，尿液和血浆中的检测需要在分析之前进行溶剂提取或其他样品处理。我们提出了一种简单的方法，通过与大环 1,4,7,10-四氮杂环十二烷-1,7-二乙酸酯 (DO2A) 螯合成铽二元络合物来定量尿液中的 SU。 SU 结合形成 [Tb(DO2A)(SU)](-) 三元络合物，由于吸光度-能量转移-发射机制，在紫外激发下引发强烈的发光。在这里，我们报告了 [Tb(DO2A)(SU)](-) 三元复合物的表征，并应用这种敏化镧系元素发光方法来量化低剂量阿司匹林方案后尿液样本中的 SU，
Salicylurate (SU) is the major metabolite in urine of acetylsalicylic acid (aspirin) and can be used as a metric to monitor aspirin pharmacokinetics and as an indicator of appendicitis, anemia, and liver disease. Detection in urine and plasma currently requires solvent extraction or other sample handling prior to analysis. We present a simple method to quantify SU in urine via chelation to a terbium binary complex with the macrocycle 1,4,7,10-tetraazacyclododecane-1,7-bisacetate (DO2A). Binding of SU to form the [Tb(DO2A)(SU)](-) ternary complex triggers intense luminescence under UV excitation due to an absorbance-energy transfer-emission mechanism. Here we report characterization of the [Tb(DO2A)(SU)](-) ternary complex and application of this sensitized lanthanide luminescence method to quantify SU in urine samples following a low-dose aspirin regimen,