Identification of tumor-restricted antigens NY-BR-1, SCP-1, and a new cancer/testis-like antigen NW-BR-3 by serological screening of a testicular library with breast cancer serum.
Identification of tumor-restricted antigens NY-BR-1, SCP-1, and a new cancer/testis-like antigen NW-BR-3 by serological screening of a testicular library with breast cancer serum.
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通过使用乳腺癌血清对睾丸文库进行血清学筛选，鉴定肿瘤限制性抗原 NY-BR-1、SCP-1 和新的癌症/睾丸样抗原 NW-BR-3。
DOI：
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发表时间：
2002
期刊：
影响因子：
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通讯作者：
A. Knuth
中科院分区：
文献类型：
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作者：
D. Jäger;M. Unkelbach;C. Frei;F. Bert;M. Scanlan;E. Jäger;L. Old;Yao;A. Knuth
重组cDNA表达文库（SEREX）的血清学分析已导致几类新的肿瘤抗原的鉴定。我们用从乳腺癌患者获得的血清分析了睾丸cDNA表达文库，并分离了命名为NW-BR-1至NW-BR-13的13个基因。其中，3个表现出肿瘤限制性表达（NW-BR-1、-2和-3），其他的被普遍表达。NW-BR-3在24个原代克隆中有9个表现出组织限制性mRNA表达，通过北方印迹检测，NW-BR-3在正常睾丸中表达，而在其他15个正常组织中不表达。RT-PCR结果显示，NW-BR-3在正常睾丸组织中有强表达，在脑、肾、气管、子宫和前列腺组织中有弱表达，在肝、心、肺、结肠、小肠、骨髓、乳腺、胸腺、肌肉、脾和胃组织中均为阴性表达。RT-PCR结果显示，NW-BR-3 mRNA在不同肿瘤组织和肿瘤细胞系中均有表达，呈现“癌/睾丸”（CT）样mRNA表达模式。NW-BR-3与从小鼠睾丸组织中克隆的小鼠基因具有71%的核苷酸和氨基酸同源性。基于mRNA表达模式，NW-BR-3代表了癌症免疫治疗的新候选靶基因。NW-BR-1和NW-BR-2也显示肿瘤限制性mRNA表达。NW-BR-1是先前由SEREX鉴定的乳腺分化抗原NY-BR-1的部分克隆。NY-BR-1在正常乳腺、睾丸和80%的乳腺癌中表达。NW-BR-2与CT抗原SCP-1相同，最初通过肾癌的SEREX分析分离。这项研究提供了进一步的证据，证明SEREX是一种强有力的工具，可以识别与免疫治疗方法潜在相关的新肿瘤抗原。
Serological analysis of recombinant cDNA expression libraries (SEREX) has led to the identification of several categories of new tumor antigens. We analyzed a testicular cDNA expression library with serum obtained from a breast cancer patient and isolated 13 genes designated NW-BR-1 through NW-BR-13. Of these, 3 showed tumor-restricted expression (NW-BR-1, -2 and -3), the others being expressed ubiquitously. NW-BR-3, representing 9 of 24 primary clones, showed tissue-restricted mRNA expression, being expressed in normal testis but not in 15 other normal tissues tested by Northern blotting. RT-PCR analysis showed strong NW-BR-3 expression in normal testis, weak expression in brain, kidney, trachea, uterus and normal prostate, and was negative in liver, heart, lung, colon, small intestine, bone marrow, breast, thymus, muscle, spleen, and stomach. NW-BR-3 mRNA expression was found in different tumor tissues and tumor cell lines by RT-PCR, thus showing a 'cancer/testis' (CT)-like mRNA expression pattern. NW-BR-3 shares 71% nucleotide and amino acid homology to a mouse gene cloned from mouse testicular tissue. Based on the mRNA expression pattern, NW-BR-3 represents a new candidate target gene for cancer immunotherapy. NW-BR-1 and NW-BR-2 also showed tumor-restricted mRNA expression. NW-BR-1 is a partial clone of the breast differentiation antigen NY-BR-1 previously identified by SEREX. NY-BR-1 is expressed in normal breast, testis and 80% of breast cancers. NW-BR-2 is identical to the CT antigen SCP-1, initially isolated by SEREX analysis of renal cancer. This study provides further evidence that SEREX is a powerful tool to identify new tumor antigens potentially relevant for immunotherapy approaches.
影响因子：
11.2
作者：
M. Disis;E. Calenoff;Graham McLaughlin;A. Murphy;Wei Chen;B. Groner;M. Jeschke;Nick Lydon;E. McGlynn;R. Livingston;R. Moe;M. Cheever
通讯作者：
M. Disis;E. Calenoff;Graham McLaughlin;A. Murphy;Wei Chen;B. Groner;M. Jeschke;Nick Lydon;E. McGlynn;R. Livingston;R. Moe;M. Cheever
从黑色素瘤互补 DNA 文库中对黑色素细胞 rab 鸟苷 5-三磷酸结合蛋白和染色体浓缩蛋白进行血清学克隆。
影响因子：
11.2
作者：
Jäger,D;Stockert,E;Jäger,E;Güre,AO;Scanlan,MJ;Knuth,A;Old,LJ;Chen,YT
通讯作者：
Chen,YT