A polysaccharide of the marine alga Capsosiphon fulvescens induces apoptosis in AGS gastric cancer cells via an IGF-IR-mediated PI3K/Akt pathway
A polysaccharide of the marine alga Capsosiphon fulvescens induces apoptosis in AGS gastric cancer cells via an IGF-IR-mediated PI3K/Akt pathway
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DOI：
10.1016/j.cellbi.2007.01.010
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发表时间：
2007-08-01
影响因子：
3.9
通讯作者：
Nam, Taek-Jeong
中科院分区：
文献类型：
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作者：
Kwon, Mi-Jin;Nam, Taek-Jeong
由于海藻提取物最近被发现具有抗氧化和抗肿瘤活性，我们分析了热水溶性多糖（PS）的海洋生物Capsosiphonfulvescens作为功能性食品的潜力，通过确定其对细胞生长和DNA合成的影响。MTS检测表明，C. fulvescens PS（Cf-PS）对体外培养的人癌细胞的增殖有明显的抑制作用，且呈剂量依赖性。Cf-PS处理的AGS细胞表现出caspase-3活化的显著增加和Bcl-2表达的降低。此外，与未处理的对照细胞相比，CfPS处理的AGS细胞中胰岛素样生长因子-I受体（IGF-IR）的磷酸化降低，这与PI 3-激酶（PI 3 K）/Akt活化一致。CfPS还降低IGF-I刺激的p85向IGF-IR和IRS-1的募集。这些结果表明Cf-PS通过抑制IGF-IR信号和PI 3 K/Akt通路抑制细胞增殖并诱导细胞凋亡。(c)2007年国际细胞生物学联合会。由爱思唯尔有限公司出版。保留所有权利。
Because seaweed extracts have recently been found to have antioxidant and anti-tumor activities, we analyzed a hot-water-soluble polysaccharide (PS) of the marine alga Capsosiphonfulvescens for its potential as a functional foodstuff by determining its effects on cell growth and DNA synthesis. MTS assays showed that the C. fulvescens PS (Cf-PS) significantly inhibited the proliferation of cultured human cancer cells in a dose-dependent manner. Cf-PS-treated AGS cells exhibited a marked increase in caspase-3 activation and a decrease in Bcl-2 expression. In addition, phosphorylation of insulin-like growth factor-I receptor (IGF-IR) was decreased in CfPS-treated AGS cells as compared to nontreated control cells, which is consistent with PI3-kinase (PI3K)/Akt activation. CfPS also decreased IGF-I-stimulated recruitment of p85 to IGF-IR and IRS-1. These results indicate that Cf-PS inhibits cell proliferation and induces apoptosis by inhibiting IGF-IR signaling and the PI3K/Akt pathway. (c) 2007 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.