INDUCIBLE GENE-EXPRESSION IN TRYPANOSOMES MEDIATED BY A PROKARYOTIC REPRESSOR
INDUCIBLE GENE-EXPRESSION IN TRYPANOSOMES MEDIATED BY A PROKARYOTIC REPRESSOR
复制标题
DOI：
10.1126/science.7761835
复制
发表时间：
1995-05-26
期刊：
影响因子：
56.9
通讯作者：
CLAYTON, C
中科院分区：
文献类型：
--
作者：
WIRTZ, E;CLAYTON, C
为原生动物寄生虫布氏锥虫开发了诱导表达系统。表达大肠杆菌四环素阻遏物的转基因锥虫表现出诱导剂（四环素）依赖性的染色体整合的报告基因的表达下的控制下的前循环酸性重复蛋白（PARP）启动子轴承fet运营商。报告基因的表达可以控制在四个数量级的范围内，响应四环素浓度，调节的程度，超过了其他真核抑制为基础的系统所表现出的。泰特阻遏物控制的PARP启动子将成为研究锥虫生物化学、致病性以及细胞和分子生物学的有用工具。
An inducible expression system was developed for the protozoan parasite Trypanosoma brucei. Transgenic trypanosomes expressing the tetracycline repressor of Escherichia coli exhibited inducer (tetracycline)-dependent expression of chromosomally integrated reporter genes under the control of a procyclic acidic repetitive protein (PARP) promoter bearing a fet operator. Reporter expression could be controlled over a range of four orders of magnitude in response to tetracycline concentration, a degree of regulation that exceeds those exhibited by other eukaryotic repression-based systems. The tet repressor-controlled PARP promoter should be a valuable tool for the study of trypanosome biochemistry, pathogenicity, and cell and molecular biology.