EFFECT OF CAROTENOIDS ON IN-VITRO IMMUNOGLOBULIN PRODUCTION BY HUMAN PERIPHERAL-BLOOD MONONUCLEAR-CELLS - ASTAXANTHIN, A CAROTENOID WITHOUT VITAMIN-A ACTIVITY, ENHANCES IN-VITRO IMMUNOGLOBULIN PRODUCTION IN RESPONSE TO A T-DEPENDENT STIMULANT AND ANTIGEN
EFFECT OF CAROTENOIDS ON IN-VITRO IMMUNOGLOBULIN PRODUCTION BY HUMAN PERIPHERAL-BLOOD MONONUCLEAR-CELLS - ASTAXANTHIN, A CAROTENOID WITHOUT VITAMIN-A ACTIVITY, ENHANCES IN-VITRO IMMUNOGLOBULIN PRODUCTION IN RESPONSE TO A T-DEPENDENT STIMULANT AND ANTIGEN
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DOI：
10.1080/01635589509514373
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发表时间：
1995-01-01
影响因子：
2.9
通讯作者：
GROSS, M
中科院分区：
文献类型：
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作者：
JYONOUCHI, H;SUN, SI;GROSS, M
采用成人志愿者和足月新生儿（脐带血）的血液样本，研究了类胡萝卜素和外周血单核细胞（PBMNC）体外免疫球蛋白（Ig）产生的影响。在类胡萝卜素补充的培养条件下，用多克隆刺激剂、新抗原和召回抗原（Ag）刺激细胞，并测量培养上清中IgM、IgA和IgG的水平。以-胡萝卜素和虾青素分别作为具有和不具有维生素A活性的类胡萝卜素的代表。虾青素对t依赖性Ag （TD-Ag）和t依赖性多克隆刺激剂的反应增强了IgM的产生。虾青素也增加了IgG的产生，以应对召回Ag。在没有补充类胡萝卜素的情况下，所有刺激物的IgA产量都可以忽略不计。然而，在类胡萝卜素补充的培养中，IgA产量对t依赖性多克隆刺激物的反应明显高于未补充的培养。10(-8) mol/l虾青素增加IgM和IgA的产生，而虾青素响应10(-10)-10(-9)mol/l的召回Ag，增加IgG的产生。在脐带血单核细胞（CBMNC）中也观察到虾青素对IgM产生的类似增强作用，尽管CBMNC产生的IgM比成人PBMNC少。β -胡萝卜素对人体Ig的产生没有显著影响。在无血清培养条件下，类胡萝卜素的作用不明显；血清对于类胡萝卜素的溶解是必不可少的。总之，这项研究首次表明虾青素，一种不含维生素a活性的类胡萝卜素，在对t依赖性刺激的反应中提高了人体的Ig产量。
The effect of carotenoids an in vitro immunoglobulin (Ig) production by peripheral blood mononuclear cells (PBMNC) was examined by employing blood samples from adult volunteers and full-term newborn babies (umbilical cord blood). Under carotenoid-supplemented culture conditions, cells were stimulated by polyclonal stimulants, neoantigens, and a recall antigen (Ag), and IgM, IgA, and IgG levels in the culture supernatant were measured. beta-Carotene and astaxanthin were used as representatives of carotenoids with and without vitamin A activity, respectively. Astaxanthin enhanced IgM production in response to T-dependent Ag (TD-Ag) and a T-dependent polyclonal stimulant.Astaxanthin also augmented IgG production in response to a recall Ag. IgA production without supplemental carotenoids was negligible for all stimuli. However, in carotenoid-supplemented cultures, IgA production was significantly higher in response to a T-dependent polyclonal stimulant than in unsupplemented cultures. IgM and IgA production was augmented at 10(-8) mol/l astaxanthin, whereas astaxanthin enhanced IgG production in response to a recall Ag at 10(-10)-10(-9) mol/l. Similar enhancing actions of astaxanthin on IgM production were observed in cord blood mononuclear cells (CBMNC), although CBMNC produced less IgM than adult PBMNC. beta-Carotene did not have a significant effect on human Ig production. The carotenoid actions were not demonstrated under serum-free culture conditions; serum is essential for solubilization of carotenoids. In summary, this study has shown for the first time that astaxanthin, a carotenoid without vitamin A activity, enhances human Ig production in response to T-dependent stimuli.