Complete nucleotide sequence of a Staphylococcus aureus exfoliative toxin B plasmid and identification of a novel ADP-ribosyltransferase, EDIN-C
Complete nucleotide sequence of a Staphylococcus aureus exfoliative toxin B plasmid and identification of a novel ADP-ribosyltransferase, EDIN-C
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DOI：
10.1128/iai.69.12.7760-7771.2001
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发表时间：
2001-12-01
影响因子：
3.1
通讯作者：
Sugai, M
中科院分区：
文献类型：
--
作者：
Yamaguchi, T;Hayashi, T;Sugai, M
测定了编码剥脱毒素B （ETB）的38.2 kb金黄色葡萄球菌质粒pETB的全核苷酸序列。在质粒基因组上共鉴定出50个开放阅读框，其中32个与已知蛋白序列相似。pETB包含三个IS257拷贝，这将pETB基因组分为三个区域：(i)耐镉操纵子含区，（ii）抗生素生产基因含区，以及（iii）质粒复制和/或维持基因分散的其余部分。在第三区域，与复制和维持相关的基因中存在各种功能的基因。它们包括两个毒力相关基因，ctb基因和一个编码与EDIN密切相关的新型adp -核糖基转移酶的基因，该基因属于修饰Rho gtpase的adp -核糖基转移酶C3家族。它们还包括细胞壁锚定表面蛋白和噬菌体抗性蛋白的基因。根据确定的pETB序列，采用PCR扫描方法对不同临床分离株携带ETB质粒（ETB质粒）的基因组结构进行分析。这些数据表明，尽管ETB质粒在基因组大小上高度异质性，但基本的基因组组织是保守的。质粒的大小变化主要归因于确定的区域，这些区域可能是基因改组的热点。
The complete nucleotide sequence of pETB, a 38.2-kb Staphylococcus aureus plasmid encoding the exfoliative toxin B (ETB), was determined. A total of 50 open reading frames were identified on the plasmid genome and, among these, 32 showed sequence similarity to known proteins. pETB contains three copies of IS257, which divide the pETB genome into three regions: (i) a cadmium resistance operon-containing region, (ii) a lantibiotic production gene-containing region, and (iii) the remaining part where genes for plasmid replication and/or maintenance are dispersed. In the third region, genes of various kinds of functions are present among the replication- and maintenance-related genes. They include two virulence-related genes, the ctb gene and a gene encoding a novel ADP-ribosyltransferase closely related to EDIN, which belongs to the C3 family of ADP-ribosyltransferases modifying Rho GTPases. They also include genes for a cell wall-anchoring surface protein and a phage resistance protein. Based on the determined sequence of pETB, the genome structures of etb-bearing plasmids (ETB plasmids) from various clinical isolates were analyzed by the PCR scanning method. The data indicate that, although the ETB plasmids are highly heterogeneous in genome size, the fundamental genome organization is well conserved. The size variation of the plasmid is mainly attributed to defined regions which may be hot spots for gene shuffling.