Assessing the role of non-cotton refuges in delaying Helicoverpa armigera resistance to Bt cotton in West Africa
Assessing the role of non-cotton refuges in delaying Helicoverpa armigera resistance to Bt cotton in West Africa
复制标题
DOI：
10.1111/j.1752-4571.2011.00207.x
复制
发表时间：
2012-01-01
影响因子：
4.1
通讯作者：
Carriere, Yves
中科院分区：
文献类型：
--
作者：
Brevault, Thierry;Nibouche, Samuel;Carriere, Yves
不含苏云金芽孢杆菌 (Bt) 毒素的非棉花宿主植物可以提供庇护所，延缓多食性害虫对 Bt 棉花的抗性。然而，事实证明，确定此类避难所的有效贡献及其在延缓抵抗演变方面的作用是困难的。在这里，我们使用生物地球化学标记来量化西非棉花带（喀麦隆）缺乏 Bt 棉花的三个农业景观中棉铃虫从非棉花宿主到棉田的移动。我们表明，非棉花宿主作为飞蛾来源的贡献在空间和时间上是可变的，但至少相当于喷洒 7.5% 的非 Bt 棉花避难所。然而，结合棉铃虫生物参数的模拟模型表明，可能需要种植非 Bt 棉花避难所，以显着延迟对产生 Cry1Ac 和 Cry2Ab 毒素的棉花的抵抗力。具体来说，当一种毒素（此处为 Cry1Ac）的浓度季节性下降时，在模拟中，对 Bt 棉花的抗性通常会迅速出现，其中非 Bt 棉花的避难所很少见，而对 Cry2Ab 的抗性是非隐性的，因为抗性本质上是由一种毒素（此处为 Cry2Ab）驱动的。使用生物地球化学标记来量化昆虫运动可以提供一个有价值的工具来评估非棉花避难所在延缓棉铃虫对 Bt 棉花抗性进化方面的作用。
Non-cotton host plants without Bacillus thuringiensis (Bt) toxins can provide refuges that delay resistance to Bt cotton in polyphagous insect pests. It has proven difficult, however, to determine the effective contribution of such refuges and their role in delaying resistance evolution. Here, we used biogeochemical markers to quantify movement of Helicoverpa armigera moths from non-cotton hosts to cotton fields in three agricultural landscapes of the West African cotton belt (Cameroon) where Bt cotton was absent. We show that the contribution of non-cotton hosts as a source of moths was spatially and temporally variable, but at least equivalent to a 7.5% sprayed refuge of non-Bt cotton. Simulation models incorporating H. armigera biological parameters, however, indicate that planting non-Bt cotton refuges may be needed to significantly delay resistance to cotton producing the toxins Cry1Ac and Cry2Ab. Specifically, when the concentration of one toxin (here Cry1Ac) declined seasonally, resistance to Bt cotton often occurred rapidly in simulations where refuges of non-Bt cotton were rare and resistance to Cry2Ab was non-recessive, because resistance was essentially driven by one toxin (here Cry2Ab). The use of biogeochemical markers to quantify insect movement can provide a valuable tool to evaluate the role of non-cotton refuges in delaying the evolution of H. armigera resistance to Bt cotton.