Electrophysiological response to herbivore-induced host plant volatiles in the moth Spodoptera littoralis
Electrophysiological response to herbivore-induced host plant volatiles in the moth Spodoptera littoralis
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DOI：
10.1046/j.1365-3032.1999.00154.x
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发表时间：
1999-12-01
影响因子：
1.5
通讯作者：
Anderson, P
中科院分区：
文献类型：
--
作者：
Jönsson, M;Anderson, P
在棉花中，棉花（锦葵科），从植物释放的挥发物的变化响应于植食性。埃及棉叶虫Spodoptera littoralis（Boisd.）（鳞翅目：夜蛾科）可以区分棉花植物之间进行幼虫喂养和未受损的植物产卵。在这项研究中，我们调查是否该蛾的女性可以检测到植食性昆虫诱导的棉花挥发物。雌性S.采用GC-EAD（coupled gaschromatography electronographic-detection）技术研究了棉小食心虫（littoralis）触角对取食棉小食心虫的挥发性物质的响应。通过GC-EAD，观察到10多种不同的棉花挥发物的反应。用单感器技术研究了刺蛾触角上短感器的反应。研究了棉小蜂对19种棉花挥发物和12种一般植物挥发物的反应。从108个受体神经元记录对这些挥发物的反应。几个神经元激活的植食性昆虫诱导的棉花挥发物被记录。例如，我们观察到对两种高萜类化合物[（E，E）-4，8，12-trimethyl-1，3，7，11-tridecatetraene和（E）-4，8-dimethyl-1，3，7-nonatriene]和（E，E）-alpha-farnesene有反应的神经元，还观察到对（+/-）-芳樟醇和吲哚有特异反应的神经元。一般来说，需要小于1 ng的刺激负荷来激活这些神经元。此外，特定的神经元被发现的组成型棉花挥发物释放与损害的植物。一个丰富的神经元类型响应β-carmillene和α-蛇麻烯。另一种类型的神经元对非诱导的棉花挥发性（Z）-茉莉酮有特异性反应。这些结果表明，S. littoralis有受体神经元，可以利用挥发性信号区分受损和未受损的植物。
In cotton, Gossypium hirsutum (Malvacae), the volatiles emitted from the plant change in response to herbivory. Ovipositing females of the Egyptian cotton leaf worm, Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) can discriminate between cotton plants subjected to larval feeding and undamaged plants during oviposition. In this study we investigate whether females of this moth can detect the herbivore-induced cotton volatiles. The response of female S. littoralis antennae to volatiles collected from cotton plants subjected to larval feeding was studied using GC-EAD (coupled gas chromatography electroantennographic-detection). By GC-EAD, responses to over 10 different cotton volatiles were observed. Using single sensillum technique the responses of short sensilla trichodea on the antennae of S. littoralis females to 19 cotton volatiles and 12 general plant volatiles were investigated. Responses to these volatiles were recorded from 108 receptor neurones. Several neurones activated by herbivore-induced cotton volatiles were recorded. For example, a neurone type responding to two homoterpenes [(E,E)-4,8,12-trimethyl-1,3,7,11-tridecatetraene and (E)-4,8-dimethyl-1,3,7-nonatriene] and (E,E)-alpha-farnesene was frequently found. We also observed sensitive neurones responding specifically to the herbivore-induced volatiles (+/-)-linalool and indole. In general, a stimulus load of less than 1 ng was needed to activate these neurones. In addition, specific neurones were found for constitutive cotton volatiles released in connection with damage to the plant. An abundant neurone type responded to beta-caryophyllene and alpha-humulene. Another neurone type responded specifically to the non-induced cotton volatile (Z)-jasmone. These results show that females of S. littoralis have receptor neurones that would make it possible to discriminate between damaged and undamaged plants using volatile signals.