Expression of telomerase catalytic component, telomerase reverse transcriptase, in human gastric carcinomas
Expression of telomerase catalytic component, telomerase reverse transcriptase, in human gastric carcinomas
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DOI：
10.1111/j.1349-7006.1998.tb00502.x
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发表时间：
1998-11-01
期刊：
影响因子：
--
通讯作者：
Tahara, E
中科院分区：
文献类型：
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作者：
Yasui, W;Tahara, H;Tahara, E
端粒酶活性被认为是细胞永生的关键，而细胞永生被认为参与了大多数人类癌症的发展。人端粒酶逆转录酶(TERT)是近年来发现的一种端粒酶催化亚单位。我们用逆转录聚合酶链式反应检测了TERT和其他端粒酶成分如人端粒酶RNA组分(hTR，由TERC编码)和人端粒酶相关蛋白(TEP1)在人胃癌和非肿瘤粘膜中的表达，并检测了端粒酶活性。在20例胃癌组织中，18例(90%)TERT表达增强，18例(90%)端粒酶活性升高，15例(75%)HTR/TERC表达增强。免疫组织化学显示，TERT蛋白在所有胃癌组织的细胞核中呈强阳性表达，而在非肿瘤性粘膜细胞及间质成分(淋巴细胞除外)中呈弱表达或阴性表达。这些发现表明，端粒酶活性相关的TERT表达增加可能成为诊断胃癌的一个新的标志物。
Telomerase activity is believed to be crucial for cellular immortality, which is considered to participate in the development of a majority of human cancers. Human telomerase reverse transcriptase (TERT) has recently been identified as a catalytic subunit of telomerase. We examined the expression of TERT and other telomerase components such as human telomerase RNA component (hTR, encoded by TERC) and human telomerase-associated protein (TEP1) by reverse transcription-polymerase chain reaction in human gastric carcinomas and non-neoplastic mucosa, in addition to measuring the telomerase activity. Of 20 gastric carcinomas examined, 18 (90%) and 18 (90%) showed increased expression of TERT and higher telomerase activity in comparison with corresponding non-neoplastic mucosa, respectively, Increased expression of hTR/TERC was also observed in 15 (75%) of the gastric carcinomas, Immunohistochemically, strong expression of TERT protein was detected in the nuclei of the tumor cells of all carcinoma tissues, while the expression of TERT in non-neoplastic mucosal cells as well as stromal elements (except lymphocytes) was weak or negative. These findings suggest that increased TERT expression associated with telomerase activity may serve as a novel marker for the diagnosis of stomach cancer.