Lipid depletion enables permeation of Staphylococcus aureus bacteria through human stratum corneum
Lipid depletion enables permeation of Staphylococcus aureus bacteria through human stratum corneum
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DOI：
10.1080/21688370.2020.1754706
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发表时间：
2020-01-01
期刊：
影响因子：
3.1
通讯作者：
German, Guy K.
中科院分区：
文献类型：
--
作者：
Lipsky, Zachary W.;Marques, Claudia N. H.;German, Guy K.
特应性皮炎（AD）是一种慢性炎症性疾病，影响全球约2-5%的成年人。阿尔茨海默病的发病机制一直是一个备受争议的猜想，提出了许多假设。阿尔茨海默病与金黄色葡萄球菌数量增加和皮肤脂质降低有关。在这项研究中，我们评估了金黄色葡萄球菌在与AD一致的正常和耗尽脂质情况下穿透人类角质层（SC）的能力。这种渗透将使细菌能够与潜在的活的表皮细胞相互作用，这可能是炎症和疾病发作的触发因素。我们的研究结果表明，金黄色葡萄球菌通过正常脂质条件的SC的渗透没有统计学意义。然而，在9天的时间内，细菌可以很容易地通过脂质耗尽的组织渗透。这些发现表明金黄色葡萄球菌可能是潜在的机制原因，而不仅仅是AD的结果。
Atopic dermatitis (AD) is a chronic inflammatory disease that affects approximately 2-5% of adults worldwide. The pathogenesis of AD continues to be a well-debated point of conjecture, with numerous hypotheses having been proposed. AD conditions are associated with increased populations of Staphylococcus aureus and reduced skin lipids. In this study, we evaluate the ability of S. aureus to permeate across human stratum corneum (SC) exhibiting both normal and depleted lipid conditions consistent with AD. This permeation would enable bacteria to interact with underlying viable epidermal cells, which could serve as a trigger for inflammation and disease onset. Our results indicate that permeation of S. aureus through SC exhibiting normal lipid conditions is not statistically significant. However, bacteria can readily permeate through lipid depleted tissue over a 9-d period. These findings suggest that S. aureus may potentially act as the mechanistic cause, rather than merely the result of AD.