Supplementary ultraviolet-B irradiation reveals differences in stress responses between Arabidopsis thaliana ecotypes
Supplementary ultraviolet-B irradiation reveals differences in stress responses between Arabidopsis thaliana ecotypes
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DOI：
10.1111/j.1365-3040.2005.01436.x
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发表时间：
2006-05-01
影响因子：
7.3
通讯作者：
Strid, Å
中科院分区：
文献类型：
--
作者：
Kalbina, I;Strid, Å
辐射拟南芥生态型C24，Wassilewskija（Ws）和哥伦比亚-0（Col-0）与补充紫外线-A + B（UV-A + B）辐射揭示了生态类型特异性差异的基因表达的发病相关蛋白PR-5。C24显示PR-5的表达水平增加（分别比Col-0和Ws高5倍和20倍）。其他分子标记物的表达，如CHS（编码查耳酮合酶）、MEB 5.2 [编码受紫外线-B（UV-B）强烈上调的基因]和PYROA [编码吡哆醇（维生素136）生物合成酶]。在不同的生态类型之间只有轻微的差异。通过H2O2染色监测UV-A + B暴露期间的氧化应激。这一分析还揭示了重要的生态类型的具体差异。在C24中发现了"H2O2热点"，而在Ws和Col-0中发现了H2O2的均匀分布。C24叶片在长时间UV-B照射后也出现坏死病变。H2 O2稳态浓度与PR-5基因表达呈负相关; Ws显示H2 O2积累水平最高，但PR-5基因表达水平最低。此外，百草枯对蟑螂的应用导致了与UV-A + B照射后发现的相似的PR-5表达和H2O2积累模式。观察到的生态差异也反映在统计上显着的UV-B依赖的生物量，莲座大小和叶面积的下降，但不是C24和Col-0。我们的研究结果表明，一个显着的生态类型特异性遗传变异的一般UV-B在拟南芥的反应存在。此外，PR-5的信号转导或基因调控途径与本研究中使用的其他分子标记物不同。
Irradiation of Arabidopsis thaliana ecotypes C24, Wassilewskija (Ws) and Columbia-0 (Col-0) with supplementary ultraviolet-A+B (UV-A+B) radiation revealed ecotype-specific differences in expression of the gene for the pathogenesis-related protein PR-5. C24 showed an increased expression level of PR-5 (5- and 20-fold higher compared with Col-0 and Ws, respectively). Expression of other molecular markers such as CHS (encoding chalcone synthase), MEB5.2 [encoding a gene strongly up-regulated by ultraviolet-B (UV-B)] and PYROA [encoding a pyridoxine (Vitamin 136) biosynthesis enzyme.] only showed slight differences between ecotypes. Oxidative stress during UV-A+B exposure was monitored by staining for H2O2. This analysis also revealed important ecotype-specific differences. 'H2O2 hot spots' were found in C24, whereas an even distribution of H2O2 was found in Ws and Col-0. Necrotic lesions also appeared on C24 leaves after prolonged UV-B exposure. There was a reverse correlation between the H2O2 steady-state concentration and the PR-5 gene expression; Ws showed the highest level of H2O2 accumulation but the lowest expression level of the PR-5 gene. Furthermore, application of paraquat on the rosettes led to similar PR-5 expression and H2O2 accumulation patterns as were found after UV-A+B irradiation. The observed ecotypic differences were also reflected in a statistically significant UV-B-dependent decrease in biomass, rosette size and leaf area for Ws, but not for C24 and Col-0. Our results show that a significant ecotype-specific genetic variability in general UV-B responses in Arabidopsis exists. Moreover, the signal transduction or gene regulation pathway for PR-5 differs from the other molecular markers used in this study.