Transcriptomic Analysis of Compromise Between Air-Breathing and Nutrient Uptake of Posterior Intestine in Loach (Misgurnus anguillicaudatus), an Air-Breathing Fish
Transcriptomic Analysis of Compromise Between Air-Breathing and Nutrient Uptake of Posterior Intestine in Loach (Misgurnus anguillicaudatus), an Air-Breathing Fish
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DOI：
10.1007/s10126-016-9713-9
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发表时间：
2016-08-01
影响因子：
3
通讯作者：
Tian, Xianchang
中科院分区：
文献类型：
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作者：
Huang, Songqian;Cao, Xiaojuan;Tian, Xianchang
道场泥鳅（Misgurnus anguillicaudatus）是一种呼吸空气的鱼类，利用后肠在水面呼吸。迄今为止，鱼类辅助空气呼吸的分子机制很少被研究。构建T01（初期组）、T02（正常组中期）、T03（正常组末期）、T04（吸气抑制中期）、T05（吸气抑制末期）5个泥鳅后肠cDNA文库，并进行RNA-seq比较其转录组图谱。总共组装了 92,962 个 unigene，同时成功注释了 37,905 个（40.77%）unigene。在T04/T02、T05/T03和T05/T04中分别观察到2298、1091和3275个差异表达基因（fn1、ACE、EGFR、Pxdn、SDF、HIF、VEGF、SLC2A1、SLC5A8等）。许多与空气呼吸和营养吸收相关的基因的表达水平在正常组和肠道空气呼吸抑制组之间存在显着差异。 T05泥鳅后肠上皮内毛细血管破裂，肠上皮内层表面红细胞富集，每毫米有241±39个细胞。正常组和空气呼吸抑制组的后肠中均存在高碘酸希夫（PAS）阳性上皮粘液细胞。这里获得的结果表明泥鳅后肠的空气呼吸和营养吸收功能存在重叠。泥鳅肠道吸气抑制会影响后肠的营养吸收能力和内皮毛细血管结构的稳定性。本研究将有助于我们了解泥鳅肠道空气呼吸的分子调控机制。
Dojo loach (Misgurnus anguillicaudatus) is an air-breathing fish species by using its posterior intestine to breathe on water surface. So far, the molecular mechanism about accessory air-breathing in fish is seldom addressed. Five cDNA libraries were constructed here for loach posterior intestines form T01 (the initial stage group), T02 (mid-stage of normal group), T03 (end stage of normal group), T04 (mid-stage of air-breathing inhibited group), and T05 (the end stage of air-breathing inhibited group) and subjected to perform RNA-seq to compare their transcriptomic profilings. A total of 92,962 unigenes were assembled, while 37,905 (40.77 %) unigenes were successfully annotated. 2298, 1091, and 3275 differentially expressed genes (fn1, ACE, EGFR, Pxdn, SDF, HIF, VEGF, SLC2A1, SLC5A8 etc.) were observed in T04/T02, T05/T03, and T05/T04, respectively. Expression levels of many genes associated with air-breathing and nutrient uptake varied significantly between normal and intestinal air-breathing inhibited group. Intraepithelial capillaries in posterior intestines of loaches from T05 were broken, while red blood cells were enriched at the surface of intestinal epithelial lining with 241 +/- 39 cells per millimeter. There were periodic acid-schiff (PAS)-positive epithelial mucous cells in posterior intestines from both normal and air-breathing inhibited groups. Results obtained here suggested an overlap of air-breathing and nutrient uptake function of posterior intestine in loach. Intestinal air-breathing inhibition in loach would influence the posterior intestine's nutrient uptake ability and endothelial capillary structure stability. This study will contribute to our understanding on the molecular regulatory mechanisms of intestinal air-breathing in loach.