Effect of CD146+ SHED on bone regeneration in a mouse calvaria defect model
Effect of CD146+ SHED on bone regeneration in a mouse calvaria defect model
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DOI：
10.1111/odi.14020
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发表时间：
2021-09-22
期刊：
影响因子：
3.8
通讯作者：
Tanimoto, Kotaro
中科院分区：
文献类型：
--
作者：
Rikitake, Kodai;Kunimatsu, Ryo;Tanimoto, Kotaro
目的人脱落乳牙（SHED）干细胞具有骨再生能力和潜在的治疗应用。 CD146是血管内皮细胞表达的细胞粘附蛋白，参与干细胞的成骨细胞分化。 CD146 对 SHED 介导的体内骨再生的影响仍不清楚。我们的目标是为 SHED 移植建立有效的条件。材料和方法 SHED 从拔出的乳牙牙髓中分离并培养；对CD146阳性(CD146(+))和CD146阴性(CD146(-))群体进行分类。将SHED、CD146(+)和CD146(-)细胞的异质群移植到免疫缺陷小鼠头骨中产生的骨缺损中。立即进行显微计算机断层扫描，并在 4 周和 8 周后进行。 8周后进行组织学和免疫组织化学评估。结果 CD146(+)和异质SHED群体移植后观察到骨再生，其中CD146(+)细胞的骨再生明显更高。 CD146(-)组的骨再生率高于对照组，但在第4周和第8周时明显低于其他移植组。组织学和免疫组织化学评估表明CD146(+)细胞促进骨再生和血管生成。结论将CD146(+)SHED移植到骨缺损处可能有助于骨再生。
Objective Stem cells from human exfoliated deciduous teeth (SHED) have bone regeneration ability and potential therapeutic applications. CD146, a cell adhesion protein expressed by vascular endothelial cells, is involved in osteoblastic differentiation of stem cells. The effect of CD146 on SHED-mediated bone regeneration in vivo remains unknown. We aimed to establish efficient conditions for SHED transplantation. Materials and methods SHED were isolated from the pulp of an extracted deciduous tooth and cultured; CD146-positive (CD146(+)) and CD146-negative (CD146(-)) populations were sorted. Heterogeneous populations of SHED and CD146(+) and CD146(-) cells were transplanted into bone defects generated in the skulls of immunodeficient mice. Micro-computed tomography was performed immediately and 4 and 8 weeks later. Histological and immunohistochemical assessments were performed 8 weeks later. Results Bone regeneration was observed upon transplantation with CD146(+) and heterogeneous populations of SHED, with significantly higher bone regeneration observed with CD146(+) cells. Bone regeneration was higher in the CD146(-) group than in the control group, but significantly lower than that in the other transplant groups at 4 and 8 weeks. Histological and immunohistochemical assessments revealed that CD146(+) cells promoted bone regeneration and angiogenesis. Conclusion Transplantation of CD146(+) SHED into bone defects may be useful for bone regeneration.