Quantitative reflection spectrophotometry: Spatial and temporal variation of Hb oxygenation in human skin
Quantitative reflection spectrophotometry: Spatial and temporal variation of Hb oxygenation in human skin
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DOI：
10.1159/000178965
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发表时间：
1995-05-01
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影响因子：
--
通讯作者：
Alexander, K
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文献类型：
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作者：
Caspary, L;Thum, J;Alexander, K
应用快速扫描反射分光光度计和多组分光谱分析，测定了 11 名健康人 8 个皮肤部位的皮肤血红蛋白氧饱和度 (S-Hb) 和相对浓度 (C-Hb)。食指尖和大脚趾的 S-Hb 显着较高（分别为 90 +/- 3.9 和 92 +/- 4.2%），而前额、前臂掌侧、手背、腹部、小腿和前足的平均值在 52% 到 67% 之间变化（p < 0.001）。肢端部位（大脚趾：2.04 +/- 0.14 任意单位 (AU)；食指：2.13 +/- 0.19 AU）的 C-Hb 也高于其他部位 (p < 0.0001)，其他部位的 C-Hb 介于 0.56 +/- 0.12 AU（腹部）和 0.95 +/- 0.28 AU（前足）之间。随着时间的推移，在 68% 的测量中观察到这两个参数以 3-5 次/分钟的频率有节律性振荡，主要是在六个近端部位。将测量部位加热至 44 摄氏度会导致 C-Hb 和 S-Hb 双相增加，这在近端部位显着 (p < 0.0001)。 S-Hb值进入动脉血范围。两个参数的时间和空间变化均减少。反射分光光度法可以直接评估真皮血红蛋白饱和度、其生理变异性以及对激发刺激的反应。与近端部位相比，真皮血管中血红蛋白的浓度和饱和度在肢端明显不同。
Applying a fast scanning reflection spectrophotometer and multicomponent spectra analysis, oxygen saturation (S-Hb) and relative concentration (C-Hb) Of hemoglobin in the skin were determined at eight skin sites in 11 healthy persons. S-Hb was significantly higher at the tip of the index finger and big toe (90 +/- 3.9 and 92 +/- 4.2%, respectively) compared with the forehead, volar forearm, back of hand, abdomen, calf and forefoot where mean values varied between 52 and 67% (p < 0.001). C-Hb also was higher at acral sites (big toe: 2.04 +/- 0.14 arbitrary units (AU); index finger: 2.13 +/- 0.19 AU) than at the other locations (p < 0.0001) where it was between 0.56 +/- 0.12 AU (abdomen) and 0.95 +/- 0.28 AU (forefoot). In the course of time, rhythmical oscillations of both parameters at a frequency of 3-5/min were seen in 68% of the measurements, predominantly at the six proximal sites. Heating the measuring site to 44 degrees C caused a biphasic increase of C-Hb and S-Hb which was significant at the proximal sites (p < 0.0001). S-Hb values came into the range of arterial blood. Temporal and spatial variation of both parameters decreased. Reflection spectrophotometry gives the possibility to directly assess dermal hemoglobin saturation, its physiological variability and reactions to provocation stimuli. Concentration and saturation of hemoglobin in dermal vessels appear definitely different at acral compared with proximal sites.