Biphasic electric current stimulates proliferation and induces VEGF production in osteoblasts
Biphasic electric current stimulates proliferation and induces VEGF production in osteoblasts
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DOI：
10.1016/j.bbamcr.2006.06.007
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发表时间：
2006-09-01
影响因子：
5.1
通讯作者：
Hwang, Soon Jung
中科院分区：
文献类型：
--
作者：
Kim, In Sook;Song, Jong Keun;Hwang, Soon Jung
本研究探讨了双相电流作为一种新型的电刺激对大鼠颅骨成骨细胞增殖、分化及细胞因子合成的影响。培养系统被设计成使得双相电流在上部和下部金板之间流动。BEC有助于在细胞暴露于电刺激期间最小化净电荷积累。将成骨细胞暴露于1.5 μ A/cm（2）、频率为3000 Hz的电刺激下，根据刺激的间隔，以间断模式（每天6小时）和连续模式（每天24小时）评估BEC的作用。而在连续模式刺激2天后增殖增加了31%，在中断模式下不受影响。成骨相关基因的转录表达，如碱性磷酸酶（ALP），骨桥蛋白和I型胶原蛋白在两种模式刺激后4天不变，而cbfa 1在相同条件下下降。促进成骨细胞分化的生长因子（BMP-2、-4、IGF-2和TGF-β 1）的mRNA表达没有检测到变化。实时荧光定量RT-PCR和ELISA结果显示BEC可显著上调血管内皮生长因子（VEGF）的表达。BEC对VEGF的诱导不是缺氧驱动的。总之，目前的体外研究表明，BEC增加细胞增殖和诱导VEGF的产生。连续刺激比间断刺激更有效。为了证实BEC是否能促进成骨，需要进一步的体内研究。(c)2006 Elsevier B. V.保留所有权利。
This study investigated biphasic electric current (BEC) functions as a new type of electrical stimulation to induce rat calvarial osteoblasts to proliferate, differentiate and synthesize cytokines. The culture system was designed so that biphasic current flowed between upper and lower gold plates. BEC helps to minimize the net charge accumulation during cell exposure to the electrical stimulation. Osteoblasts were exposed to electrical stimulation of 1.5 mu A/cm(2) at 3000 Hz, and the effect of BEC was assessed in the interrupted mode (6 h daily) and in the continuous mode (24 h daily), depending on the interval of stimulation. Whereas proliferation increased by 31% after stimulation in the continuous mode for 2 days, it was unaffected in the interrupted mode. The transcriptional expression of osteogenesis-related genes such as alkaline phosphatase (ALP), osteopontin, and type I collagen was unchanged 4 days after stimulation in both modes, while cbfa1 was decreased under the same conditions. There was no detectable change in mRNA expression of growth factors (BMP-2, -4, IGF-2 and TGF-beta 1) that promote osteoblast differentiation. However, real-time RT-PCR and ELISA demonstrated that vascular endothelial growth factor (VEGF) was markedly up-regulated by BEC. Induction of VEGF by BEC was not hypoxia driven. In conclusion, the present in vitro study demonstrates that BEC increases cell proliferation and induces the production of VEGF. The BEC was more effective with continuous stimulation than with interrupted stimulation. To confirm whether BEC can enhance osteogenesis, further in vivo studies are needed. (c) 2006 Elsevier B.V. All rights reserved.