The effect of freezing and long-term storage on the stability of cardiac troponin T
The effect of freezing and long-term storage on the stability of cardiac troponin T
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DOI：
10.1309/lr7fc0luglht8x6j
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发表时间：
2007-07-01
影响因子：
3.5
通讯作者：
Maciejko, James J.
中科院分区：
文献类型：
--
作者：
Basit, Majid;Bakshi, Nasir;Maciejko, James J.
心肌肌钙蛋白T （cTnT）水平被广泛用于评估心肌梗死的证据。我们研究了冷冻和长期储存对178例终末期肾衰竭患者血液样本中cTnT稳定性的影响。将血清分离并分成多个等分。在未冷冻的等分液中测量基线cTnT水平。剩下的等分用标准技术冷冻。在3个月、6个月、12个月或24个月后解冻等分，并测量cTnT水平。与基线（0.114 +/- 0.098 mu g/L）相比，12个月的平均SEM cTnT水平（0.111 +/- 0.098 mu g/L）没有显著变化；24个月后，cTnT水平显著降低(0。107 0.095 μ g/L)比基线高（P = 0.004）。cTnT测定是一种可靠的方法，可测量人血清中长达12个月的冷冻储存的cTnT水平。然而，24个月后，cTnT水平为0。比基线低007 μ g/L，可能导致错误解释。长期冷冻保存后cTnT水平变化的临床意义尚不清楚，进一步的研究，包括对患者结果的前瞻性分析，应该会有所帮助。
Cardiac troponin T (cTnT) levels are widely used to assess for evidence of myocardial infarction. We studied the effect offreezing and long-term storage on the stability of cTnT in blood samples from 178 patients with end-stage renal failure. The serum was separated and divided into multiple aliquots. Baseline cTnT levels were measured in the unfrozen aliquots. The remaining aliquots were frozen using standard techniques. The aliquots were thawed after 3, 6, 12, or 24 months and cTnT levels measured.There were no significant changes in the mean SEM cTnT levels up to 12 months (0.111 +/- 0.098 mu g/L) compared with baseline (0.114 +/- 0.098 mu g/L); after 24 months, cTnT levels were significantly lower (0. 107 0.095 mu g/L) than baseline (P =.004). The cTnT assay is a reliable method of measuring the cTuT level in human serum up to 12 months offrozen storage. However, after 24 months, the cTnT level was 0. 007 mu g/L lower than baseline, potentially causing erroneous interpretations. The clinical significance of the change in the cTnT level after long-termfrozen storage is unclear Further studies, including prospective analysis of patient outcomes, should be helpful.